Diagnosis of cytomegaloviral pneumonia by in situ hybridization.
Diagnosis of cytomegaloviral pneumonia by in situ hybridization.
复制标题
原位杂交诊断巨细胞病毒肺炎。
DOI：
10.1093/infdis/150.2.272
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Meyers,JD
中科院分区：
文献类型：
--
作者：
Myerson,D;Hackman,RC;Meyers,JD
用原位杂交法对29例开胸肺活检标本进行巨细胞病毒检测。将生物素标记的巨细胞病毒DNA与福尔马林固定、石蜡包埋的组织切片杂交，用免疫过氧化物酶程序检测。该技术的敏感性与病毒培养和冰冻切片中的病毒抗原检测的敏感性相似。通过对福尔马林固定的、石蜡包埋的肺组织切片的研究，在24小时内诊断出巨细胞病毒感染。该分析具有特异性、重复性好、易于应用于用于诊断目的的多组织切片研究和回溯性研究。对两例肺炎死亡病例的多个切片的杂交研究表明，巨细胞病毒感染并不是所有病例都是均匀的。
Cytomegalovirus was assayed by in situ hybridization in 29 consecutive specimens obtained by open-lung biopsy. Biotin-labeled cytomegaloviral DNA was hybridized to formalin-fixed, paraffin-embedded tissue sections and was detected by an immunoperoxidase procedure. The sensitivity of this technique was similar to that of both viral culture and viral antigen detection in frozen sections by monoclonal antibody. Cytomegaloviral infection was diagnosed within 24 hr by study of the formalin-fixed, paraffin-embedded sections of lung tissue. The assay was specific, reproducible, and readily applicable both to the study of multiple tissue sections for diagnostic purposes and to retrospective studies. Hybridization studies of multiple sections from two patients with fatal cases of pneumonia showed that cytomegaloviral infection is not homogeneous in all cases.