BASE-PAIRING INVOLVING DEOXYINOSINE - IMPLICATIONS FOR PROBE DESIGN
BASE-PAIRING INVOLVING DEOXYINOSINE - IMPLICATIONS FOR PROBE DESIGN
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DOI：
10.1093/nar/13.24.8927
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发表时间：
1985-12-20
影响因子：
14.9
通讯作者：
TINOCO, I
中科院分区：
文献类型：
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作者：
MARTIN, FH;CASTRO, MM;TINOCO, I
用光学熔解技术测定了含有与四种正常DNA碱基相匹配的脱氧肌醇（1）残基的寡脱氧核苷酸双链体的热稳定性。含有至少一个I的双链体通过将等摩尔量的序列dCA 3XA 3G的寡核苷酸与序列dCT 3 YT 3G之一混合而获得，其中X和Y是A、C、G、T或I。光学熔解曲线的比较产生了相对稳定性的I-containing的标准碱基对，否则相同的碱基对序列。在这些双链体中，发现I：C对不如A：T对稳定。观察到大的邻基效应对稳定性的影响。例如，当（X，Y）=（I，A）时，双链体比当（X，Y）=（A，I）时稳定8倍。与序列效应无关，稳定性的顺序为：I：C > I：A > I：T = I：G。该顺序不同于脱氧鸟苷的顺序，脱氧鸟苷与dA的配对不太强烈;否则，在相同序列环境中，每个脱氧-肌苷碱基对比其脱氧鸟苷对应物更不稳定。这些结果的DNA寡核苷酸探针的设计的影响进行了讨论。
The thermal stability of oligodeoxyribonucleotide duplexes containing deoxyinoslne (1) residues matched with each of the four normal DNA bases were determined by optical melting techniques. The duplexes containing at least one I were obtained by nixing equimolar amounts of an oligonucleotide of sequence dCA3XA3G with one of sequence dCT3YT3G where X and Y were A, C, G, T, or I. Comparison of optical melting curves yielded relative stabilities for the I-containlng standard base pairs in an otherwise identical base-pair sequence. I:C pairs were found to be less stable than A:T pairs in these duplexes. Large neighboring-base effects upon stability were observed. For example, when (X,Y)=(I,A), the duplex is eight-fold more stable than when (X,Y)=(A,I). Independent of sequence effects the order of stabilities is: I:C > I:A > I:T = I:G. This order differs from that of deoxyguanosine which pairs less strongly with dA; otherwise each deoxy-lnosine base pair is less stable than its deoxyguanosine counterpart in the same sequence environment. Implications of these results for design of DNA oligonucleotide probes are discussed.