Measurement of two-bond JCOHα coupling constants in proteins uniformly enriched with13C
Measurement of two-bond JCOHα coupling constants in proteins uniformly enriched with13C
复制标题
均匀富集 13 C 的蛋白质中双键 JCOHα 偶联常数的测量
DOI：
10.1007/bf01874818
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发表时间：
1992
影响因子：
2.7
通讯作者：
A. Bax
中科院分区：
文献类型：
--
作者：
G. W. Vuister;A. Bax
本文介绍了一种简单的E.COSY型技术，用于测定均匀富集13 C的蛋白质中的双键JCOHα偶合常数。该方法已用于测量蛋白质钙调素和葡萄球菌核酸酶中具有非重叠Hα−Cα相关性的132个残基的2JCOH α。测得的2JCOH α耦合常数在0至−9.5 Hz范围内。一个单独的实验，测量这些值的准确性，表明均方根误差为1 Hz。将J耦合与晶体学研究中的二面角背骨角进行比较，证实二面角θ与2JCOH α的大小之间存在微弱但统计学显著的相关性，但也表明除了θ之外的参数对耦合值有显著影响。
SummaryA simple E.COSY type technique is described for measurement of two-bond JCOHα coupling constants in proteins that are uniformly enriched with13C. The method has been used to measure2JCOHα for 132 residues in the proteins calmodulin and staphylococcal nuclease having non-overlapping Hα−Cα correlations. Measured2JCOHα coupling constants fall in the 0 to −9.5 Hz range. A separate experiment, measuring the accuracy of these values, indicates a root-mean-square error of 1 Hz. Comparison of the J couplings with the dihedral back bone angles from crystallographic studies confirms a weak but statistically significant correlation between the dihedral angle ψ and the magnitude of2JCOHα, but also indicates that parameters other than ψ have a significant effect on the value of the coupling.
影响因子：
5.6
作者：
BABU, YS;BUGG, CE;COOK, WJ
通讯作者：
COOK, WJ