Analysis of protein kinases by Phos-tag SDS-PAGE
Analysis of protein kinases by Phos-tag SDS-PAGE
复制标题
通过 Phos-tag SDS-PAGE 分析蛋白激酶
DOI：
10.1016/j.jprot.2022.104485
复制
发表时间：
2022
影响因子：
3.3
通讯作者：
Uezato Yuuki
中科院分区：
文献类型：
--
作者：
Sugiyama Yasunori;Uezato Yuuki
蛋白激酶调节几乎所有的生物过程，包括细胞增殖、分化、凋亡和基因表达。由蛋白激酶的异常活性和表达引起的蛋白磷酸化的失调导致各种疾病如癌症和代谢综合征的发作。许多蛋白激酶的活性受磷酸化调节。因此，分析蛋白激酶的磷酸化状态对于阐明生物现象和疾病的发病机制具有重要意义。为了研究蛋白质磷酸化，开发了磷酸结合标签分子“Phos-tag”。此外，已经开发了使用Phos标签的各种技术和工具，如Phos标签SDS-PAGE，用于蛋白质磷酸化的分析和分析。本文主要介绍了Phos标记蛋白激酶磷酸化的方法和分析技术，以及磷酸化分析在蛋白激酶研究中的应用。测定蛋白激酶的磷酸化状态可以为它们的活化提供必要的信息。本文综述了Phos标记的SDS-PAGE分析技术在蛋白激酶磷酸化状态分析中的应用。我们相信这篇评论将有助于读者进行运动组学研究。
Protein kinases regulate almost all biological processes including cell proliferation, differentiation, apoptosis, and gene expression. Dysregulation of protein phosphorylation caused by abnormal activity and expression of protein kinases results in the onset of various diseases such as cancer and metabolic syndromes. The activities of a large number of protein kinases are regulated by phosphorylation. Therefore, analysis of the phosphorylation status of protein kinases is important for elucidation of biological phenomena and the pathogenesis of diseases. To investigate protein phosphorylation, phosphate-binding tag molecule “Phos-tag” was developed. In addition, various techniques and tools using Phos-tag such as Phos-tag SDS-PAGE, have been developed for analysis and profiling of protein phosphorylation. Here, we describe the methods and analytical techniques that use Phos-tag for investigation of protein kinase phosphorylation and the applications of phosphorylation analysis.SignificanceProtein kinases play pivotal roles in regulating many biological processes and pathogenesis of diseases. Determination of phosphorylation status of protein kinases can provide the essential information for their activation. This review provides analytical techniques for analysis of phosphorylation status of protein kinases by Phos-tag SDS-PAGE. We believe that this review would help readers to study in kinomics research.