Curcumin Ameliorates Memory Deficits by Enhancing Lactate Content and MCT2 Expression in APP/PS1 Transgenic Mouse Model of Alzheimer's Disease
Curcumin Ameliorates Memory Deficits by Enhancing Lactate Content and MCT2 Expression in APP/PS1 Transgenic Mouse Model of Alzheimer's Disease
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姜黄素通过增强阿尔茨海默病 APP/PS1 转基因小鼠模型中的乳酸含量和 MCT2 表达来改善记忆缺陷
DOI：
10.1002/ar.23969
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发表时间：
2019
影响因子：
2
通讯作者：
Huang Juan
中科院分区：
文献类型：
--
作者：
Lu Wei Tian;Sun Shan Quan;Li Yu;Xu Shi Ye;Gan Sheng Wei;Xu Jin;Qiu Guo Ping;Zhuo Fei;Huang Si Qin;Jiang Xu Li;Huang Juan
姜黄素是一种天然产物，具有多种抗阿尔茨海默病（AD）神经保护特性。本研究旨在探讨姜黄素对APP/PS1 AD小鼠模型记忆障碍、乳酸含量和单羧酸转运体2（MCT 2）的影响。本研究使用APP/PS1转基因小鼠和野生型（WT）C57 BL/6 J小鼠。采用Morris水迷宫实验检测小鼠的空间学习记忆能力。采用乳酸测定法测定大脑皮层和海马乳酸含量。免疫组化和Western blotting检测大脑皮质和海马中MCT 2的表达。结果显示，与APP/PS1小鼠组相比，姜黄素处理的APP/PS1小鼠组的空间学习和记忆障碍得到改善。姜黄素处理的APP/PS1小鼠的脑乳酸含量和MCT 2蛋白水平高于APP/PS1小鼠。总之，我们的研究结果表明，姜黄素可以改善AD的APP/PS1小鼠模型的记忆障碍。这种现象可能至少部分是由于其对脑中乳酸含量和MCT 2蛋白表达的改善作用。Anat Rec，302：332-338，2019。© 2018威利期刊股份有限公司
Curcumin is a natural product with several anti‐Alzheimer's disease (AD) neuroprotective properties. This study aimed to investigate the effects of curcumin on memory deficits, lactate content, and monocarboxylate transporter 2 (MCT2) in APP/PS1 mouse model of AD. APP/PS1 transgenic mice and wild‐type (WT) C57BL/6J mice were used in the present study. Spatial learning and memory of the mice was detected using Morris water‐maze test. Cerebral cortex and hippocampus lactate contents were detected using lactate assay. MCT2 expression in the cerebral cortex and hippocampus was examined by immunohistochemistry and Western blotting. Results showed that spatial learning and memory deficits were improved in curcumin‐treated APP/PS1 mouse group compared with those in APP/PS1 mice group. Brain lactate content and MCT2 protein level were increased in curcumin‐treated APP/PS1 mice than in APP/PS1 mice. In summary, our findings indicate that curcumin could ameliorate memory impairments in APP/PS1 mouse model of AD. This phenomenon may be at least partially due to its improving effect on the lactate content and MCT2 protein expression in the brain. Anat Rec, 302:332–338, 2019. © 2018 Wiley Periodicals, Inc.