Three-Dimensional Analysis of Collagen Lamellae in the Anterior Stroma of the Human Cornea Visualized by Second Harmonic Generation Imaging Microscopy
Three-Dimensional Analysis of Collagen Lamellae in the Anterior Stroma of the Human Cornea Visualized by Second Harmonic Generation Imaging Microscopy
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DOI：
10.1167/iovs.10-5657
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发表时间：
2011-02-01
影响因子：
4.4
通讯作者：
Nishida, Teruo
中科院分区：
文献类型：
--
作者：
Morishige, Naoyuki;Takagi, Yuki;Nishida, Teruo
目的。人角膜前基质中的胶原层结构被认为是决定角膜硬度的重要因素。方法从Descemet‘s剥离自动内皮角膜移植术(Descemet’s Stripping Automated EndothalKeratplating，DSAEK)的供体组织中获取27个正常人角膜的前部，并用二次谐波成像显微镜(SHG)观察中央角膜块(类似于3 mm方形)。从Bowman‘s层表面扫描每个角膜，扫描深度150微米，采集SHG前向信号。测量紧邻下方至Bowman层以下30微米处的胶原层(缝合层)的夹角，以及50或100微米处的胶原层夹角。结果：前基质中的胶原层分布均匀，方向随机。缝合椎板相对于Bowman‘s层的夹角为19.19±4.34°(平均+/-SD)。50目和100目深度的胶原层夹角分别为8.91±2.91°和6.91±2.11°。缝合层密度为910.0+/-480.4/mm(2)，其宽度分别为13.14+/-5.03和7.11+/-3.00亩。结论正常人角膜前基质中的胶原层是三维交织的，并与鲍曼层紧密结合。这种结构可能导致角膜前部的僵硬和弯曲。(投资眼科VS科学。2011年；52：911-915)doi：10.1167/iovs.10-5657
PURPOSE. The structure of collagen lamellae in the anterior stroma of the human cornea is thought to be an important determinant of corneal rigidity. The three-dimensional structure of such collagen lamellae in normal human corneas was examined.METHODS. The anterior portion of 27 normal human corneas was obtained from donor tissue for Descemet's stripping automated endothelial keratoplasty (DSAEK) surgery, and blocks (similar to 3-mm square) of the central cornea were examined by second harmonic generation (SHG) imaging microscopy. Each cornea was scanned from the surface of Bowman's layer to a depth of 150 mu m, and SHG forward signals were collected. The angles of collagen lamellae immediately below to a depth of 30 mu m below Bowman's layer (sutural lamellae) as well as of those at a depth of 50 or 100 mu m were measured. The density and width of sutural lamellae were also evaluated.RESULTS. Collagen lamellae in the anterior stroma were evenly distributed and randomly oriented. The angle of sutural lamellae relative to Bowman's layer was 19.19 +/- 4.34 degrees (mean +/- SD). The angles of collagen lamellae at depths of 50 or 100 mu m were 8.91 +/- 2.91 and 6.91 +/- 2.11 degrees, respectively. The density of sutural lamellae was 910.0 +/- 480.4/mm(2), and their width was 13.14 +/- 5.03 and 7.11 +/- 3.00 mu m in the region immediately beneath and 30 mu m below Bowman's layer, respectively.CONCLUSIONS. Collagen lamellae in the anterior stroma of the normal human cornea are interwoven in three dimensions and adhere densely to Bowman's layer. This structure may contribute to the rigidity and curvature of the anterior portion of the cornea. (Invest Ophthalmol Vis Sci. 2011;52:911-915) DOI:10.1167/iovs.10-5657