Species Diversity of the Genus Ulva (Ulvophyceae, Chlorophyta) in Japanese Waters, with Special Reference to Ulva tepida Masakiyo et S. Shimada sp. nov.
Species Diversity of the Genus Ulva (Ulvophyceae, Chlorophyta) in Japanese Waters, with Special Reference to Ulva tepida Masakiyo et S. Shimada sp. nov.
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发表时间：
2014-02
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通讯作者：
Yuka Masakiyo;S. Shimada
中科院分区：
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作者：
Yuka Masakiyo;S. Shimada
为了准确了解日本Ulva和Umbraulva （ulvophysiae，绿藻门）的物种多样性，对日本水域从30 m左右的深海海水、沿海地区、咸淡水到淡水的164个Ulva和Umbraulva标本进行了nrITS2区测序。系统发育分析表明，日本乌尔瓦属植物可分为29个不同的分支，包括17个已知种和12个具有新序列的种。日本伞属植物共分为4种，其中2种具有新序列。Ulva sp. 5与Ulva clathratioides Kraft等在大体形态和细胞内类pyrenoids数量上形态相似，两者之间仅相差1 bp。Ulva sp. 12具有膜质菌体，在基部的细胞层上有延伸的根状体，这一特征仅在Segawa Ulva subittoralis报道过。Ulva tepida Masakiyo et S.Shimada sp. 11 . （Ulva sp. 3）的特点是：管状菌体在基部有单根或径向分支，叶绿体覆盖细胞外壁，中部有1-5个类pyrenoids（1, 15.6%; 2, 54.1%; 3, 25.9%; 4, 3.7%; 5, 0.7%）。
In order to understand the exact species diversity of Japanese Ulva and Umbraulva (Ulvophyceae, Chlorophyta), nrITS2 region was sequenced from 164 specimens of Ulva and Umbraulva collected in Japanese waters from deep seawater around 30 m depth, coastal areas, brackish water to freshwater. Phylogenetic analysis revealed that Japanese Ulva fall into 29 distinct clades that composed of 17 known species and 12 species possessing novel sequences. Japanese Umbraulva was classified into four species, two of which possessed novel sequences. Ulva sp. 5 was morphologically similar to Ulva clathratioides Kraft et al. in gross morphology and number of pyrenoids in cells, and there was only 1 bp difference between them. Ulva sp. 12 had membranous thalli with rhizoids extending the outside on the cell layer in the basal portion, a characteristic reported only from Ulva sublittoralis Segawa. Ulva tepida Masakiyo et S.Shimada sp. nov. (Ulva sp. 3) is characterized by tube-like thalli with simple or radial branching in the basal region, chloroplasts covering the outer wall of cells and having 1–5 pyrenoids (one, 15.6%; two, 54.1%; three, 25.9%; four, 3.7%; and five, 0.7%) in the middle region.