Quercetin inhibits virulence properties of Porphyromas gingivalis in periodontal disease.
Quercetin inhibits virulence properties of Porphyromas gingivalis in periodontal disease.
复制标题
槲皮素抑制牙周病中牙龈卟啉菌的毒力特性
DOI：
10.1038/s41598-020-74977-y
复制
发表时间：
2020-10-27
影响因子：
4.6
通讯作者：
Zhou W
中科院分区：
文献类型：
--
作者：
He Z;Zhang X;Song Z;Li L;Chang H;Li S;Zhou W
牙龈卟啉单胞菌是牙周病发生和进展的病原体。本研究旨在研究槲皮素（一种天然植物产品）对牙龈卟啉单胞菌毒力特性（包括牙龈蛋白酶、血凝素和生物膜形成）的影响。检测槲皮素对牙龈卟啉单胞菌的抗菌作用和形态变化。使用显色肽和绵羊红细胞评价槲皮素对牙龈蛋白酶活性以及溶血、血凝活性的影响。通过结晶紫和MTT测定评估不同浓度槲皮素的生物膜生物量和代谢。通过共焦激光扫描显微镜观察生物膜的结构和厚度。还评估了细菌细胞表面特性，包括细胞表面疏水性和聚集。使用实时 PCR 评估毒力和铁/血红素利用的 mRNA 表达。槲皮素具有抗菌作用并破坏细胞结构。槲皮素可以在亚 MIC 浓度下抑制牙龈蛋白酶、溶血、血凝活性和生物膜形成。分子对接分析进一步表明槲皮素可以与牙龈蛋白酶相互作用。槲皮素处理后生物膜变得稀疏且变薄。槲皮素还调节细胞表面疏水性和聚集。在槲皮素存在下，测试基因的表达下调。总之，我们的研究表明槲皮素可抑制牙龈卟啉单胞菌的多种毒力因子。
Porphyromonas gingivalis is a causative agent in the onset and progression of periodontal disease. This study aims to investigate the effects of quercetin, a natural plant product, on P. gingivalis virulence properties including gingipain, haemagglutinin and biofilm formation. Antimicrobial effects and morphological changes of quercetin on P. gingivalis were detected. The effects of quercetin on gingipains activities and hemolytic, hemagglutination activities were evaluated using chromogenic peptides and sheep erythrocytes. The biofilm biomass and metabolism with different concentrations of quercetin were assessed by the crystal violet and MTT assay. The structures and thickness of the biofilms were observed by confocal laser scanning microscopy. Bacterial cell surface properties including cell surface hydrophobicity and aggregation were also evaluated. The mRNA expression of virulence and iron/heme utilization was assessed using real time-PCR. Quercetin exhibited antimicrobial effects and damaged the cell structure. Quercetin can inhibit gingipains, hemolytic, hemagglutination activities and biofilm formation at sub-MIC concentrations. Molecular docking analysis further indicated that quercetin can interact with gingipains. The biofilm became sparser and thinner after quercetin treatment. Quercetin also modulate cell surface hydrophobicity and aggregation. Expression of the genes tested was down-regulated in the presence of quercetin. In conclusion, our study demonstrated that quercetin inhibited various virulence factors of P. gingivalis.
登录
查看更多内容
影响因子：
3.1
作者：
CHU, L;BRAMANTI, TE;HOLT, SC
通讯作者：
HOLT, SC
影响因子：
4.6
作者：
Guevara, Tibisay;Rodriguez-Banqueri, Arturo;Xavier Gomis-Ruth, F.
通讯作者：
Xavier Gomis-Ruth, F.
影响因子：
3.1
作者：
Aruni, A. Wilson;Lee, J.;Fletcher, H. M.
通讯作者：
Fletcher, H. M.
影响因子：
3
作者：
Dixon, Douglas R.;Jeffrey, Nicole R.;Leung, Kai P.
通讯作者：
Leung, Kai P.
影响因子：
5.6
作者：
Casado-Diaz, Antonio;Anter, Jaouad;Manuel Quesada-Gomez, Jose
通讯作者：
Manuel Quesada-Gomez, Jose