THYMINE GLYCOL AND THYMIDINE GLYCOL IN HUMAN AND RAT URINE - A POSSIBLE ASSAY FOR OXIDATIVE DNA DAMAGE
THYMINE GLYCOL AND THYMIDINE GLYCOL IN HUMAN AND RAT URINE - A POSSIBLE ASSAY FOR OXIDATIVE DNA DAMAGE
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DOI：
10.1073/pnas.81.18.5633
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
AMES, BN
中科院分区：
文献类型：
--
作者：
CATHCART, R;SCHWIERS, E;AMES, BN
胸腺嘧啶乙二醇是电离辐射和其他氧化诱变剂的DNA损伤产物。为了找到一种非侵入性的测定个体中的氧化性DNA损伤，开发了一种HPLC [高效液相色谱]测定尿液中游离胸腺嘧啶乙二醇和胸苷乙二醇的方法。人类排泄. apprx. 32 nmol的2种二醇/天。大鼠具有较高的比代谢率和较短的寿命，其排泄物约为0.000000。胸腺嘧啶乙二醇加胸苷乙二醇/kg体重是人类的15倍。胸腺嘧啶乙二醇和胸苷乙二醇可能来自氧化DNA的修复，而不是来自其他来源，如饮食或细菌植物群。这种DNA氧化产物的非侵入性测定可能允许直接测试目前的理论，这些理论将氧化代谢与人类衰老和癌症的过程联系起来。
Thymine glycol is a DNA damage product ionizing radiation and other oxidative mutagens. To find a noninvasive assay for oxidative DNA damage in individuals, a HPLC [high performance liquid chromatography] assay was developed for free thymine glycol and thymidine glycol in urine. Humans excrete .apprx. 32 nmol of the 2 glycols/day. Rats, which have a higher specific metabolic rate and shorter life span, excrete .apprx. 15 times more thymine glycol plus thymidine glycol/kg of body wt than do humans. Thymine glycol and thymidine glycol are likely to be derived from repair of oxidized DNA, rather than from alternative sources such as the diet or bacterial flora. This noninvasive assay of DNA oxidation products may allow the direct testing of current theories which relate oxidative metabolism to the processes of aging and cancer in man.