Eugenol modifies the excitability of rat sciatic nerve and superior cervical ganglion neurons
Eugenol modifies the excitability of rat sciatic nerve and superior cervical ganglion neurons
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DOI：
10.1016/j.neulet.2010.02.009
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发表时间：
2010-03-26
影响因子：
2.5
通讯作者：
Leal-Cardoso, Jose H.
中科院分区：
文献类型：
--
作者：
Moreira-Lobo, Daniel C. A.;Linhares-Siqueira, Emmanuely D.;Leal-Cardoso, Jose H.
丁香酚是一种苯丙烯，从丁香和罗勒等植物的精油中提取，在牙科中有广泛的用途。丁香酚具有镇痛作用，可能与抑制电压依赖性Na+通道和/或激活TRPV1受体或两者有关。在电流箝位条件下，用尖锐微电极刺穿离体大鼠坐骨神经和颈上神经节（SCG）神经元的复合动作电位，获得电生理参数。丁香酚对离体大鼠坐骨神经复合动作电位的抑制呈浓度依赖性。从SCG神经元记录的动作电位被丁香酚抑制，IC50为0.31 mM。在高浓度（2 mM），在短暂的应用。丁香酚对静息膜电位和膜输入电阻无干扰，但对动作电位有明显的阻断作用。然而，令人惊讶的是，在低丁香酚浓度（0.6 mM）下，在长时间应用中，观察到输入膜电阻的可逆降低（约50%），这表明丁香酚可能参与了降低神经元兴奋性的二次延迟效应。2010爱思唯尔爱尔兰有限公司版权所有。
Eugenol is a phenylpropene obtained from the essential oils of plants such as clove and basil which has ample use in dentistry. Eugenol possesses analgesic effects that may be related to the inhibition of voltage-dependent Na+ channels and/or to the activation of TRPV1 receptors or both. In the present study, electrophysiological parameters were taken from the compound action potentials of the isolated rat sciatic nerve and from neurons of the superior cervical ganglion (SCG) impaled with sharp microelectrodes under current-clamp conditions. In the isolated rat sciatic nerve, eugenol inhibited the compound action potential in a concentration-dependent manner. Action potentials recorded from SCG neurons were inhibited by eugenol with an IC50 of 0.31 mM. At high concentrations (2 mM), during brief applications. eugenol caused significant action potential blockade while it did not interfere with the resting membrane potential or the membrane input resistance. Surprisingly, however, at low eugenol concentrations (0.6 mM), during long time applications, a reversible reduction (by about 50%) in the input membrane resistance was observed, suggesting the possible involvement of a secondary delayed effect of eugenol to reduce neuronal excitability. (C) 2010 Elsevier Ireland Ltd. All rights reserved.