Analysis of single nanoparticle detection by using 3-dimensionally confined optofluidic ring resonators
Analysis of single nanoparticle detection by using 3-dimensionally confined optofluidic ring resonators
复制标题
使用 3 维受限光流控环形谐振器进行单纳米颗粒检测分析
DOI：
10.1364/oe.18.025081
复制
发表时间：
2010-11-22
期刊：
影响因子：
3.8
通讯作者：
Fan, Xudong
中科院分区：
文献类型：
--
作者：
Li, Hao;Guo, Yunbo;Fan, Xudong
病毒颗粒是大多数人类致命疾病的罪魁祸首，包括埃博拉热、流感、艾滋病毒、非典、登革热等。这些半径在1 nm到800 nm之间的小型传染性病原体，其传播和传播速度极快，如果人类被感染，治疗时间非常有限[1]。这些疾病的预防和早期诊断需要快速、微量检测液体和空气中的病毒。在采用的许多方法中，基于光学环形谐振器的生物传感器是最灵敏的设备之一，能够以实时且无标记的方式检测单个病毒体或纳米颗粒[2-3]。在环形谐振器中，光循环并形成回音壁模式 (WGM)。当病毒颗粒或纳米颗粒结合到谐振器表面时，其与 WGM 的相互作用会导致光谱偏移或模式分裂 [2-3]。迄今为止，通过测量波长偏移，已经用固体微球实验检测到了液体中的单个流感颗粒（半径为 50 nm）[2]。最近，通过测量模式分裂，空气中单个纳米颗粒（半径 30 nm）的检测和尺寸测定也已通过微型环形线圈进行了演示 [3]。然而，尽管它们具有出色的传感性能，但这两种结构都缺乏有效的流体系统来将样品快速输送到传感头（即环形谐振器），这可能会显着延长检测时间，特别是在检测单个纳米颗粒时。
Viral particles are responsible for the majority of human fatal diseases, including Ebola fever, influenza, HIV, SARS, dengue fever, and so on. Those small infectious agents with radius ranging from 1 nm to 800 nm spread and transmit extremely rapidly, and leave very limited time for treatment if humans are infected [1]. The prevention and early diagnosis of those diseases require fast and trace amount detection of virus in liquid and in air. Among many approaches employed, the optical ring resonator based biosensor is one of the most sensitive devices, capable of detecting a single virion or nanoparticle in a real-time and label-free manner [2–3]. In a ring resonator, light circulates and forms whispering-gallery modes (WGMs). When a virion or nanoparticle binds onto the resonator surface, its interaction with the WGM leads to a spectral shift or mode splitting [2–3]. To date, by measuring the wavelength shift, a single influenza particle (50 nm in radius) in liquid has been detected experimentally with a solid microsphere [2]. Recently, by measuring the mode splitting, the detection and sizing of a single nanoparticle (30 nm in radius) in air have also been demonstrated with a microtoroid [3]. However, despite their excellent sensing performance, both structures lack of an efficient fluidic system to rapidly deliver samples to the sensing head (i.e., the ring resonator), which may significantly lengthen the detection time, in particular, when detecting a single nanoparticle.