MOLECULAR-ORGANIZATION OF THE HLA-SB REGION OF THE HUMAN MAJOR HISTOCOMPATIBILITY COMPLEX AND EVIDENCE FOR 2 SB BETA-CHAIN GENES
MOLECULAR-ORGANIZATION OF THE HLA-SB REGION OF THE HUMAN MAJOR HISTOCOMPATIBILITY COMPLEX AND EVIDENCE FOR 2 SB BETA-CHAIN GENES
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DOI：
10.1073/pnas.81.13.3934
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
MACH, B
中科院分区：
文献类型：
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作者：
GORSKI, J;ROLLINI, P;MACH, B
主要组织相容性复合体的II类产物，也称为Ia抗原，由2条多肽链组成。和度量。链，都在主要组织相容性复合体内编码。在人类中，II类抗原可分为3个生物化学上不同的组，分别是HLA-DR、HLA-DC和HLA-SB。多态β的互补DNA克隆的分离。HLA-DR和HLA-DC的链使得研究II类基因的组织成为可能。HLA-SB。从同源纯合子b细胞系生成的cosmid文库中，在重组克隆中鉴定出-chain基因。SB。beta。-链基因与SB . α相连接。-链基因和2个基因的取向相反。第二个SB。beta。-链基因，对应于新的SB . β。II基因座也得到了鉴定和克隆。SB。beta。-链基因的等位基因限制性位点多态性比-链基因少得多。HLA-DR或HLA-DC链。
The class II products of the major histocompatibility complex, also called Ia antigens, are composed of 2 polypeptide chains, the .alpha. and .beta. chains, both encoded within the major histocompatibility complex. In man, the class II antigens can be divided into 3 biochemically distinct groups called HLA-DR, HLA-DC and HLA-SB. Isolation of c[complementary]DNA clones for the polymorphic .beta. chain of HLA-DR and HLA-DC has allowed the study of the organization of the class II genes. The HLA-SB .beta.-chain gene was identified in recombinant clones from a cosmid library generated from a consanguineous homozygous B-cell line. The SB .beta.-chain gene is linked to the SB .alpha.-chain gene and the 2 genes are in opposite orientation. A 2nd SB .beta.-chain gene, corresponding to a new SB .beta. II locus, was also identified and cloned. The SB .beta.-chain genes show much less allelic restriction site polymorphism than the genes for the .beta. chains of HLA-DR or HLA-DC.