Expression of protein kinase C isozymes in rat glial cell line.
Expression of protein kinase C isozymes in rat glial cell line.
复制标题
蛋白激酶C同工酶在大鼠神经胶质细胞系中的表达。
DOI：
10.1097/00001756-199304000-00023
复制
发表时间：
1993
期刊：
影响因子：
1.7
通讯作者：
Madhukar,B
中科院分区：
文献类型：
--
作者：
Kumar,K;Kim,B;Rupp,H;Madhukar,B
为了研究神经胶质细胞中PKC的各种同工酶的存在和分布，对来自大鼠大脑皮层的皮层神经胶质细胞系培养物进行免疫荧光研究，并通过高灵敏度的自动激光细胞仪ACAS-570（粘附细胞分析和分选）进行分析。检测的抗体针对PKC的[α]、[β]、[γ]和e同工酶，以及催化结构域（cd），即针对PKC所有同工酶的cd的抗体。这些数据表明，大鼠皮质胶质细胞系表达至少三种PKC同工酶，[β]和[α]，免疫荧光强度的程度>[β]>[α]。PKC-cd的免疫荧光最强。
To study the presence and distribution of various isozymes of PKC in glial cells, immunofluorescent studies were performed on a cortical glial cell line culture derived from rat cerebral cortex and analyzed by a highly sensitive automated laser cytometric device, ACAS-570 (Adherent Cell Analysis and Sorting). The antibodies tested were against [alpha],[beta],[gamma], and e isozymes of PKC, and the catalytic domain (cd), ie antibody against the cd to all isozymes of PKC. The data indicate that rat cortical glial cell line expresses at least three isozymes of PKC,[beta], and [alpha], the degree of intensity of immunofluorescence being>[beta]>[alpha]. The immunofluorescence for PKC-cd was the most intense.