STREAMING-Tag System: Technology to Enable Visualization of Transcriptional Activity and Subnuclear Localization of Specific Endogenous Genes
STREAMING-Tag System: Technology to Enable Visualization of Transcriptional Activity and Subnuclear Localization of Specific Endogenous Genes
复制标题
STREAMING-Tag 系统：实现特定内源基因转录活性和亚核定位可视化的技术
DOI：
10.1007/978-1-0716-2724-2_8
复制
发表时间：
2022
影响因子：
--
通讯作者：
Ochiai Hiroshi
中科院分区：
文献类型：
--
作者：
Ohishi Hiroaki;Ochiai Hiroshi
剪接TetO REpeAt、MS 2重复序列和IN蛋白夹心报告基因标签（STREAMING-标签）系统能够在活细胞中以低于100 nm的分辨率成像特定内源基因的核定位以及转录活性。使用该系统结合成像的表观基因组状态，使详细分析的影响表观基因组状态的转录动力学。在本章中，我们描述了一种方法，用于定量Nanogene和簇的辅因子BRD 4使用STREAMING标签系统在小鼠胚胎干细胞之间的距离。
The Spliced TetO REpeAt, MS2 repeat, and INtein sandwiched reporter Gene tag (STREAMING-tag) system enables imaging of nuclear localization as well as the transcription activity of a specific endogenous gene at sub-100-nm resolution in living cells. The use of this system combined with imaging of epigenome states enables a detailed analysis of the impact of epigenome status on transcriptional dynamics. In this chapter, we describe a method for quantifying distances betweenNanoggene and clusters of cofactor BRD4 using the STREAMING-tag system in mouse embryonic stem cells.