Difference of Serum Cytokine Profile in Allergic Asthma Patients According to Disease Severity.
Difference of Serum Cytokine Profile in Allergic Asthma Patients According to Disease Severity.
复制标题
过敏性哮喘患者血清细胞因子谱随疾病严重程度的差异
DOI：
10.2147/jaa.s345759
复制
发表时间：
2022
影响因子：
3.2
通讯作者：
Jin M
中科院分区：
文献类型：
--
作者：
Sun L;Peng B;Zhou J;Wang P;Mo Y;Xu G;Tao Y;Song H;Tang W;Jin M
目的变应性哮喘是一种异质性疾病，其发病机制复杂。细胞因子是免疫系统中的关键介质，是疾病状态的潜在指标。本研究的目的是比较不同疾病严重程度的过敏性哮喘患者血清细胞因子谱的差异，探索疾病监测和靶向治疗药物的候选生物标志物。患者和方法共40例过敏性哮喘患者（轻度，n=22;中重度，n=18）纳入本研究。从每个受试者收集血清样本、肺功能和呼出的一氧化氮数据。应用Meso Scale Discovery（MSD）电化学发光平台来获得33种细胞因子的血清水平。比较轻、中、重度过敏性哮喘患者血清细胞因子水平，分析血清细胞因子水平与肺功能、呼出气一氧化氮的相关性。结果中重度过敏性哮喘患者血清中eotaxin-1、eotaxin-2、MCP-1、MCP-2、MCP-3、YKL-40水平高于轻度过敏性哮喘患者，而IL-23、IL-31、TRAIL水平低于轻度过敏性哮喘患者。血清YKL-40、eotaxin-1和MCP-1对轻度和中重度过敏性哮喘的鉴别能力最强，AUC分别为0.833、0.811和0.760。血清IP-10与FeNO水平呈正相关，而FnNO与血清IL-25呈强正相关。结论与轻度过敏性哮喘患者相比，中重度过敏性哮喘患者血清eotaxin-1、eotaxin-2、MCP-1、MCP-2、MCP-3、YKL-40水平明显升高，IL-23、IL-31、TRAIL水平明显降低。YKL-40、Eotaxin-1和MCP-1可能是反映过敏性哮喘患者病情严重程度的候选生物标志物。
Purpose Allergic asthma is a heterogeneous disease with complex underlying mechanisms. Cytokines are key mediators in immune system and potential indicators of disease status. The aim of this study is to compare the difference of serum cytokine profile in allergic asthma patients with different disease severity and explore candidate biomarkers for disease monitoring and targeting therapeutic agents. Patients and Methods A total of 40 allergic asthmatics (mild, n=22; moderate-to-severe, n=18) were included in this study. Serum samples, lung function and exhaled nitric oxide data were collected from each subject. A Meso Scale Discovery (MSD) electrochemiluminescence platform was applied to access serum levels of 33 cytokines. Serum cytokine profile was compared between mild and moderate-to-severe allergic asthmatics, and the correlation between serum cytokine levels, lung function and exhaled nitric oxide were analyzed. Results Moderate-to-severe allergic asthmatics displayed higher levels of eotaxin-1, eotaxin-2, MCP-1, MCP-2, MCP-3, YKL-40 and lower IL-23, IL-31 and TRAIL in serum in comparison with mild allergic asthmatics. Serum YKL-40, eotaxin-1 and MCP-1 had the best ability to discriminate mild and moderate-to-severe allergic asthmatics, with an AUC of 0.833, 0.811 and 0.760. Serum IP-10 was positively correlated with FeNO levels, while FnNO displayed a strong positive correlation with serum IL-25. Conclusion Compared with mild allergic asthmatics, significant increase in serum eotaxin-1, eotaxin-2, MCP-1, MCP-2, MCP-3, YKL-40 and decrease in serum IL-23, IL-31 and TRAIL was noted in moderate-to-severe allergic asthmatics. YKL-40, eotaxin-1 and MCP-1 might be candidate biomarkers in reflecting severity in allergic asthma patients.