Breeding of YY super-male of blotched snakehead (Channa maculata) and production of all-male hybrid (Channa argus ? ? C. maculata ?)
Breeding of YY super-male of blotched snakehead (Channa maculata) and production of all-male hybrid (Channa argus ? ? C. maculata ?)
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斑鳢（Channa maculata）YY超级雄性的选育和全雄性杂交种（Channa argus ? ? C. maculata ?）的生产
DOI：
10.1016/j.aquaculture.2021.736450
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发表时间：
2021
期刊：
影响因子：
4.5
通讯作者：
Chen Kunci
中科院分区：
文献类型：
--
作者：
Zhao Jian;Ou Mi;Wang Yaping;Liu Haiyang;Luo Qing;Zhu Xinping;Chen Baixiang;Chen Kunci
许多商业上重要的鱼类在生长和大小上表现出显著的性别二型性。养殖单性种能提高养殖户的经济效益。黑鱼在中国大量养殖，每年超过50万吨。乌鳢的性别二型性显著，雄性个体的生长和大小平均是雌性的2倍。此外，可销售的鱼的个体大小也决定了价格。因此，全雄群体在黑鱼养殖产业中具有巨大的利益。本研究采用性反转和分子性别鉴定相结合的方法，培育出YY超雄性斑点鳢，并通过YY超雄性C的种间杂交培育出全雄性杂交种。斑鳢与XX头正常雌性北方乌鳢（Channa argus）杂交，用于水产养殖。用α-雌二醇、β-雌二醇和己烯雌酚三种激素诱导C.斑点。所有激素诱导性逆转的效果相似，且逆转率呈剂量依赖性。所有激素处理组均出现卵巢和输卵管异常，导致自然产卵失败。因此，人工授精是繁殖新雌性的必要条件。利用性连锁标记对XY新雌体后代进行基因分型，成功检测到YY超雄候选个体，但与预期的1：2：1比例存在偏差（χ2= 6.17，df = 2，P< 0.05）。YY超雄性候选者进一步产生一半的YY超雄性和一半的XY正常雄性与XY新雌性，以及100%的雄性与XX正常雌性C。斑点。用YY超级雄性不育系C进行杂交，获得了全雄杂种后代。斑点病与XX例正常女性C. argus。以正常杂种为对照，对全雄杂种的生长性能进行了比较。在同一土池中饲养120 d后，全雄杂交种日增重比对照组日增重4.88 g/d提高5.73 g/d，提高17.3%。全雄杂种的性比经随机抽样评估为93%雄性，并且在意外的雌性的性腺中出现两性组织。本研究结果证明了乌鳢全雄鱼繁殖的可行性，并可推广到其他乌鳢养殖中。
Many commercially important fish species show significant sex dimorphism in growth and size. Breeding mono-sex stocks could improve economic benefits of farmers. The snakehead fish is massively cultured in China, over 0.5 million tons per year. The sex dimorphism of snakehead is remarkable, in which males are meanly twice in growth and size than females. Furtherly, the individual size of the marketable fish also determines the price. So the all-male stock is of great beneficial interests in the snakehead culture industry. In this study, we combined the sex reversal and molecular sex identification to produce YY super-male blotched snakehead,Channamaculata, and then bred the all-male hybrid by an interspecific hybridization of YY super-maleC. maculatawith XX normal female northern snakehead (Channa argus) for aquaculture. Three kinds of hormones,α-estradiol,β-estradiol, and stilbestrol, were used to induce sex reversal ofC. maculata. All hormones resulted in similar effects in inducing sex reversal, and the reversal rate were dosage dependent. Abnormal ovaries and oviducts were found in all hormone treated groups, which resulted in failure of natural spawning. Thus, artificial insemination was essential in the reproduction of neofemale. The sex-linked marker was used to genotype the progeny of XY neofemale, and YY super-male candidates with new genotypes were successfully detected, but biased from the expected 1:2:1 ratio (χ2= 6.17, df = 2,P< 0.05). The YY super-male candidates furtherly produced half of YY super-males and half of XY normal males with XY neofemales, and 100% males with XX normal femaleC. maculata. All-male hybrid stock was also produced by crossing the YY super-maleC. maculatawith XX normal femaleC. argus. The growth performance of the all-male hybrid was compared with the normal hybrid as control. After 120 days feeding in the same earthen ponds, the all-male hybrid gained 5.73 g/d in average daily gain (ADG) compared to 4.88 g/d of the control, about 17.3% improved. The sex ratio of the all-male hybrid was evaluated by random sampling to be 93% male, and the bisexual tissue emerged in the gonad of the unexpected female. The results of this research prove that the all-male breeding of snakehead fish is practicable and would be applied in other snakehead fish.