GAL4 drivers specific for Type Ib and Type Is motor neurons in Drosophila
GAL4 drivers specific for Type Ib and Type Is motor neurons in Drosophila
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果蝇 Ib 型和 Is 型运动神经元特异的 GAL4 驱动程序
DOI：
10.1101/445577
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Perez-Moreno J
中科院分区：
文献类型：
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作者：
Perez-Moreno J
果蝇黑腹幼虫神经肌肉系统被研究人员广泛用于神经细胞生物学研究，果蝇谷氨酸能运动神经元已成为主要的模型系统。谷氨酸能运动神经元主要有Ib和IS两种类型，在神经肌肉接头的突触水平上具有不同的结构和生理特性。为了产生基因工具来识别和操纵每种类型的运动神经元，我们为此目的筛选了GAL4驱动系。在这里，我们描述了特定于每种类型、Ib或IS中的神经元的例子的GAL4驱动程序。这些驱动程序显示出高水平的表达，并且只在少数运动神经元中表达，使它们成为在已识别的I型运动神经元中进行轴突和突触生物学的特定研究的工具。
TheDrosophila melanogasterlarval neuromuscular system is extensively used by researchers to study neuronal cell biology, andDrosophilaglutamatergic motor neurons have become a major model system. There are two main Types of glutamatergic motor neurons, Ib and Is, with different structural and physiological properties at synaptic level at the neuromuscular junction. To generate genetic tools to identify and manipulate motor neurons of each Type, we screened forGAL4driver lines for this purpose. Here we describeGAL4drivers specific for examples of neurons within each Type, Ib or Is. These drivers showed high expression levels and were expressed in only few motor neurons, making them amenable tools for specific studies of both axonal and synapse biology in identified Type I motor neurons.