GENOMIC AND CDNA CLONES OF THE HOMEOTIC LOCUS ANTENNAPEDIA IN DROSOPHILA
GENOMIC AND CDNA CLONES OF THE HOMEOTIC LOCUS ANTENNAPEDIA IN DROSOPHILA
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DOI：
10.1002/j.1460-2075.1983.tb01696.x
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发表时间：
1983-01-01
期刊：
影响因子：
11.4
通讯作者：
GEHRING, WJ
中科院分区：
文献类型：
--
作者：
GARBER, RL;KUROIWA, A;GEHRING, WJ
同源异型基因参与了发育途径的控制：例如，果蝇触角基因座的显性突变导致果蝇头部的触角被中胸腿取代。采用染色体步移和跳跃相结合的方法，从果蝇中克隆了一个含有果蝇足的DNA区域。五个DNA倒位重排，这是与mannapedia突变体表型被定位在一个25 kb的区域内。该区域的基因组DNA序列被用作杂交探针，以筛选从果蝇胚胎和蛹poly（A）+ RNA制备的c[互补]DNA文库。分离出一个2.2 kb的cDNA序列（903），它似乎来自至少4个跨越100 kb的非连续染色体区域。它包括反转断点的位置。2.9kb的第二cDNA（909）由来自至少3个染色体区域的序列组成，其中2个与903克隆中包含的序列相似或相同，但第三个来自推定的903内含子内的基因组DNA。这个轨迹的不寻常的大小和复杂性进行了讨论。
Homeotic genes are involved in the control of developmental pathways: dominant mutations at the Antennapedia locus of Drosophila, for example, lead to replacement of the antennae on the head of the fly by mesothoracic legs. A combination of chromosome walking and jumping were used to clone a DNA region from Drosophila containing Antennapedia. Five DNA inversion rearrangements which are associated with the Antennapedia mutant phenotype were localized within a 25-kb [kilobase] region. Genomic DNA sequences from this area were used as hybridization probes to screen c[complementary]DNA libraries prepared from Drosophila embryonic and pupal poly(A)+ RNA. A 2.2-kb cDNA sequence (903) was isolated which appears to derive from at least 4 non-contiguous chromosomal regions that span 100 kb. It includes the positions of the inversion breakpoints. A 2nd cDNA of 2.9 kb (909) is composed of sequences from at least 3 chromosomal regions, 2 of which are similar or identical to sequences contained in the 903 clone but the 3rd is derived from genomic DNA within a putative 903 intron. The unusual size and complexity of this locus are discussed.