Photolysis of Hydrogen Peroxide, an Effective Disinfection System via Hydroxyl Radical Formation
Photolysis of Hydrogen Peroxide, an Effective Disinfection System via Hydroxyl Radical Formation
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DOI：
10.1128/aac.00751-10
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发表时间：
2010-12-01
影响因子：
4.9
通讯作者：
Kohno, Masahiro
中科院分区：
文献类型：
--
作者：
Ikai, Hiroyo;Nakamura, Keisuke;Kohno, Masahiro
研究了H2O2光解产生的羟基自由基的量与杀菌活性之间的关系。用波长405nm的激光照射H2O2(1M)，产生羟基自由基。电子自旋共振自旋捕获分析表明，羟基自由基的产生量随着照射时间的延长而增加。杀菌试验中使用了四种致病性口腔细菌：金黄色葡萄球菌、伴放线聚合杆菌、变形链球菌和粪肠球菌。还检查了模型生物膜中的变形链球菌。对 1 M H2O2 中的悬浮液进行激光照射，在处理 3 分钟内，每个测试物种的活菌计数减少了 99.99% 以上。对生物膜中的变形链球菌进行处理后，3 分钟内活菌计数减少了 99.999% 以上。其他结果表明，杀菌活性取决于产生的羟基自由基的量。用 200 至 300 μM 羟基自由基处理细菌将导致活菌计数减少 >99.99%。
The relationship between the amount of hydroxyl radicals generated by photolysis of H2O2 and bactericidal activity was examined. H2O2 ( 1 M) was irradiated with laser light at a wavelength of 405 nm to generate hydroxyl radicals. Electron spin resonance spin trapping analysis showed that the amount of hydroxyl radicals produced increased with the irradiation time. Four species of pathogenic oral bacteria, Staphylococcus aureus, Aggregatibacter actinomycetemcomitans, Streptococcus mutans, and Enterococcus faecalis, were used in the bactericidal assay. S. mutans in a model biofilm was also examined. Laser irradiation of suspensions in 1 M H2O2 resulted in a >99.99% reduction of the viable counts of each of the test species within 3 min of treatment. Treatment of S. mutans in a biofilm resulted in a >99.999% reduction of viable counts within 3 min. Other results demonstrated that the bactericidal activity was dependent on the amount of hydroxyl radicals generated. Treatment of bacteria with 200 to 300 mu M hydroxyl radicals would result in reductions of viable counts of >99.99%.