Dissecting the ATP hydrolysis pathway of bacterial enhancer-binding proteins
Dissecting the ATP hydrolysis pathway of bacterial enhancer-binding proteins
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DOI：
10.1042/bst0360083
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发表时间：
2008-02-01
影响因子：
3.9
通讯作者：
Zhang, Xiaodong
中科院分区：
文献类型：
--
作者：
Bose, Daniel;Joly, Nicolas;Zhang, Xiaodong
bebp（细菌增强子结合蛋白）是AAA+（与各种细胞活动相关的atp酶）转录激活因子，通过特定的细菌sigma因子sigma激活基因转录（54）。sigma(54)-RNAP （RNA聚合酶）与启动子DNA位点结合，形成稳定的封闭复合物，无法进行转录。封闭的复合体必须利用bebp提供的ATP水解能量进行重塑，以熔化DNA并启动转录。最近，大量的结构和生化数据已经对bebp活性位点内ATP水解如何与封闭复合物的重新建模相耦合产生了见解。在本文中，我们回顾了一些关键的核苷酸、突变和使用的技术，以及它们如何有助于我们理解bebp的功能。
bEBPs (bacterial enhancer -binding proteins) are AAA+ (ATPase associated with various cellular activities) transcription activators that activate gene transcription through a specific bacterial sigma factor, sigma(54). sigma(54)-RNAP (RNA polymerase) binds to promoter DNA sites and forms a stable closed complex, unable to proceed to transcription. The closed complex must be remodelled using energy from ATP hydrolysis provided by bEBPs to melt DNA and initiate transcription. Recently, large amounts of structural and biochemical data have produced insights into how ATP hydrolysis within the active site of bEBPs is coupled to the re-modelling of the closed complex. In the present article, we review some of the key nucleotides, mutations and techniques used and how they have contributed towards our understanding of the function of bEBPs.