Cloning of the cDNA for a new member of the immunoglobulin superfamily (ISLR) containing leucine-rich repeat (LRR)
Cloning of the cDNA for a new member of the immunoglobulin superfamily (ISLR) containing leucine-rich repeat (LRR)
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DOI：
10.1006/geno.1997.4889
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发表时间：
1997-09-15
期刊：
影响因子：
4.4
通讯作者：
Shimizu, N
中科院分区：
文献类型：
--
作者：
Nagasawa, A;Kubota, R;Shimizu, N
我们分离了一种新的蛋白质的cDNA，计算分子量为46 kDa，含有一个富含亮氨酸的重复序列（LRR）与保守的侧翼序列和C2型免疫球蛋白（IG）样结构域。这种新蛋白被认为是IG超家族的新成员，被命名为ISLR（immunoglobulin superfamily containing LRR）。已知这些结构域对于蛋白质-蛋白质相互作用或细胞粘附是重要的，因此新蛋白质ISLR也可能与其他蛋白质或细胞相互作用。北方印迹分析显示在各种人类组织中存在2.4-kb转录物，包括视网膜、心脏、骨骼肌、前列腺、卵巢、小肠、甲状腺、肾上腺皮质、睾丸、胃和脊髓以及胎儿肺和胎儿肾。应用荧光原位杂交技术将ISLR基因定位于人染色体15 q23-q24。(C)北京：科学出版社.
We isolated cDNAs for a novel protein with a calculated molecular mass of 46 kDa, containing a leucine-rich repeat (LRR) with conserved flanking sequences and a C2-type immunoglobulin (Ig)-like domain. This novel protein was considered to be a new member of the Ig superfamily and was named ISLR (immunoglobulin superfamily containing LRR). These domains are known to be important for protein-protein interaction or cell adhesion, and therefore it is possible that the novel protein ISLR may also interact with other proteins or cells. Northern blot analysis showed the presence of a 2.4-kb transcript in various human tissues including retina, heart, skeletal muscle, prostate, ovary, small intestine, thyroid, adrenal cortex, testis, stomach, and spinal cord as well as fetal lung and fetal kidney. The ISLR gene was mapped on human chromosome 15q23-q24 by fluorescence in situ hybridization. (C) 1997 Academic Press.