Fluorescence polarization of tetracycline derivatives as a technique for mapping nonmelanoma skin cancers
Fluorescence polarization of tetracycline derivatives as a technique for mapping nonmelanoma skin cancers
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DOI：
10.1117/1.2435710
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发表时间：
2007-01-01
影响因子：
3.5
通讯作者：
Flotte, Thomas
中科院分区：
文献类型：
--
作者：
Yaroslavsky, Anna N.;Salomatina, Elena V.;Flotte, Thomas
非黑色素瘤皮肤癌是人类癌症中最常见的形式，通常导致高发病率。这些肿瘤的低视觉对比度使得它们的描绘成为一个具有挑战性的问题。采用线偏振单色光源和宽视场CCD相机，我们已经开发出一种技术，用于荧光偏振成像的非黑色素瘤癌症染色使用抗生素从四环素家族。为了确定该方法的可行性，研究了86个厚的新鲜癌症切除物的荧光偏振图像。我们发现，内源性荧光偏振的水平远低于外源性，四环素衍生物的荧光偏振的平均值显着高于癌组织相比，正常组织。在86个肿瘤中[54个在地美环素（DMN）中染色，32个在四环素（TCN）中染色]，在79个病例中（51-DMN，28-TCN）准确地识别了病变的位置、大小和形状。这项试验的结果表明，非黑色素瘤皮肤肿瘤可以区分健康组织的基础上的差异，外源性荧光偏振的TCN和/或DMN。因此，所开发的技术可以为图像引导的癌症手术提供重要的新工具。(c)2007年，由光学仪器工程师协会（Society of Photo-Optical Instrumentation Engineers）主办。
Nonmelanoma skin cancer is the most common form of human cancer, often resulting in high morbidity. Low visual contrast of these tumors makes their delineation a challenging problem. Employing a linearly polarized monochromatic light source and a wide-field CCD camera, we have developed a technique for fluorescence polarization imaging of the nonmelanoma cancers stained using antibiotics from the tetracycline family. To determine the feasibility of the method, fluorescence polarization images of 86 thick, fresh cancer excisions were studied. We found that the level of endogenous fluorescence polarization was much lower than that of exogenous, and that the average values of fluorescence polarization of tetracycline derivatives were significantly higher in cancerous as compared to normal tissue. Out of 86 tumors [54 stained in demeclocycline (DMN) and 32 in tetracycline (TCN)], in 79 cases (51-DMN, 28-TCN) the location, size, and shape of the lesions were identified accurately. The results of this trial indicate that nonmelanoma skin tumors can be distinguished from healthy tissue based on the differences in exogenous fluorescence polarization of TCN and/or DMN. Therefore, the developed technique can provide an important new tool for image-guided cancer surgery. (c) 2007 Society of Photo-Optical Instrumentation Engineers.