Virulence-associated sequence duplication at the hemagglutinin cleavage site of avian influenza viruses
Virulence-associated sequence duplication at the hemagglutinin cleavage site of avian influenza viruses
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DOI：
10.1016/s0168-1702(97)01468-8
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发表时间：
1997-06-01
期刊：
影响因子：
5
通讯作者：
Senne, D
中科院分区：
文献类型：
--
作者：
Perdue, ML;Garcia, M;Senne, D
来自墨西哥的禽流感（Al）病毒的最新高致病性（HP）田间分离株都在血凝素（HA）糖蛋白的切割位点处具有至少两个碱性氨基酸（arg-lys）的插入。一个HP分离株具有产生4个氨基酸插入物（arg-lys-arg-lys）的额外信息。我们在这里提出的HA基因的核苷酸序列，这种独特的分离株，并比较它最近的H5 N2和其他禽流感分离株。该分离株HA基因全序列和系统发育关系表明，它是从一个非致病性分离株直接继承约1个月前。独特的插入序列是在HA切割位点处精氨酸密码子之前的富含嘌呤的区域的一部分的直接复制。该证据沿着本报告中的其他数据，为解释A型流感病毒中自发性、毒力相关插入机制的拟议模型提供了令人信服的支持。(C)1997年Elsevier Science B.V.
Recent highly pathogenic (HP) field isolates of avian influenza (Al) virus from Mexico all possess an insertion of at least two basic amino acids (arg-lys) at the cleavage site of the hemagglutinin (HA) glycoprotein. One HP isolate has additional information which yields a 4 amino acid insert (arg-lys-arg-lys). We present here the nucleotide sequence of the HA gene of this unique isolate and compare it to recent H5N2 and other avian influenza isolates. The complete HA nucleotide sequence of the isolate and phylogenetic relationship suggest that it was derived in direct succession from a non-pathogenic strain isolated about 1 month earlier. The unique insertion sequence is a direct duplication of part of the purine-rich region preceding the arginine codon at the HA cleavage site. This evidence along with other data in this report provide compelling support for a proposed model explaining the mechanism of spontaneous, virulence-related insertions in type A influenza viruses. (C) 1997 Elsevier Science B.V.