Expression of Indian hedgehog during fracture healing in adult rat femora
Expression of Indian hedgehog during fracture healing in adult rat femora
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DOI：
10.1007/pl00005843
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发表时间：
2000-04-01
影响因子：
4.2
通讯作者：
Noda, M
中科院分区：
文献类型：
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作者：
Murakami, S;Noda, M
印度刺猬蛋白（Ihh）最近被证明在胚胎发育过程中的肥大前和肥大软骨细胞中表达，并且与软骨细胞终末分化的调节有关。在本文中，我们研究了成年大鼠模型骨折愈合过程中 Ihh 的表达。右股骨横向骨干骨折，于骨折后1、2、3周检测骨折愈伤组织中Ihh的表达。 Northern 印迹分析证明了这些组织中 Ihh mRNA 的表达。骨折后1周，免疫组织学分析在骨折愈伤组织的肥大前软骨细胞中检测到hedgehog蛋白。从两周起，肥大软骨细胞中也观察到阳性染色。第3周时，一些靠近软骨内骨化前沿的成骨细胞也被染色为刺猬蛋白阳性。我们的数据表明，在成年大鼠股骨骨折愈合过程中，Ihh 在软骨细胞和成骨细胞中表达，这表明 Ihh 作为胚胎发育中软骨内骨化的调节剂，也可能在成年动物骨折修复过程中的骨形成调节中发挥作用。
Indian hedgehog (Ihh) has recently been shown to be expressed in prehypertrophic and hypertrophic chondrocytes during embryonic development, and it has been implicated in the regulation of terminal differentiation of chondrocytes. In this paper we examined the expression of Ihh during fracture healing in an adult rat model. A transverse diaphyseal fracture was made in the right femur, and the expression of Ihh in the fracture callus was examined at 1, 2, and 3 weeks after fracture. Northern blot analysis demonstrated the expression of Ihh mRNA in these tissues. Immunohistological analysis detected hedgehog protein in prehypertrophic chondrocytes in the fracture callus at 1 week after fracture. From 2 weeks and on, positive staining was observed in hypertrophic chondrocytes as well. At 3 weeks, some of the osteoblasts close to the endochondral ossification front were also stained positive for hedgehog protein. Our data indicate that Ihh is expressed in chondrocytes and osteoblasts during the process of fracture healing in adult rat femora, suggesting that Ihh, a regulator of endochondral ossification in embryonic development, may also play a role in the regulation of bone formation during fracture repair in adult animals.