Two RFLPs identified by a human chromosome #2 clone at 2p15-2p16 (HGM8 assignment no. D2S5).
Two RFLPs identified by a human chromosome #2 clone at 2p15-2p16 (HGM8 assignment no. D2S5).
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由人类染色体识别的两个 RFLP
DOI：
10.1093/nar/13.14.5403
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发表时间：
1985
影响因子：
14.9
通讯作者：
Latt,SA
中科院分区：
文献类型：
--
作者：
Shiloh,Y;Kanda,N;Kunkel,LM;Bruns,G;Sakai,K;Latt,SA
克隆的来源和描述：1.85 kb 单拷贝片段在 pBR322 中亚克隆，该片段来自从 Hind III 文库分离的重组噬菌体（在 Charon 21A 中完全消化），由流式分选染色体 1 号 DNA 制成。图 1 包含来自人神经母细胞瘤 IMR-32 的均质染色区域 (HSR)。该片段在 HSR (1) 中扩增了 75 倍。 多态性：1) EcoRI (G/AATTC)(New England Biolabs) 鉴定出 6.0 kb 处的一个不变带和 23.0 或 15.0 kb 处带的两个等位基因多态性。 2) MspI (C/CGG)(New England Biolabs) 鉴定出 11.0 kb 处的一个不变条带以及 6. 8 kbor、6. 1 和 0.7 kb 处条带的两个等位基因多态性。
SOURCE AND DESCRIPTION OF CLONE: 1.85 kb single copy fragment subcloned in pBR322 from a recombinant phage isolated from a Hind III library (complete digest in Charon 21A) made with DNA of flow sorted chromosome no. 1 containing a homogeneouslystaining region (HSR) from a human neuroblastoma, IMR-32. This fragment is amplified 75 fold in that HSR (1).POLYMORPHISMS: 1) EcoRI (G/AATTC)(New England Biolabs) identifies one invariant band at 6.0 kb and a two allele polymorphism with a band at either 23.0 or 15.0 kb. 2) MspI (C/CGG)(New England Biolabs) identifies one invariant band at 11.0 kb and a two allele polymorphism with bands at either 6. 8 kbor 6. 1 and 0.7 kb.