Differential ability of human immunodeficiency virus isolates to productively infect human cells.
Differential ability of human immunodeficiency virus isolates to productively infect human cells.
复制标题
人类免疫缺陷病毒分离株有效感染人类细胞的差异能力。
DOI：
10.4049/jimmunol.138.10.3415
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发表时间：
1987
影响因子：
4.4
通讯作者：
J. Levy
中科院分区：
文献类型：
--
作者：
L. Evans;T. McHugh;D. Stites;J. Levy
HIV分离株在连续的人类造血细胞系中复制的能力表现出明显的差异。此外，尽管从健康个体获得的所有PMC培养物都可以感染HIV，但观察到从不同PMC培养物释放的病毒量存在相当大的差异。HIV的这些生物学特性与临床状态、病毒分离株与靶细胞的结合特性或靶细胞CD 4抗原表达的差异无关。一些不能直接感染HUT-78细胞系的HIV分离株在将感染这些病毒的PMC加入该人T细胞系时显示出生产性感染。这些观察结果强调了细胞与细胞接触在病毒传播中的重要性。结果首次证明了几种单独PMC培养物中HIV分离株的宿主范围特异性的差异，并表明HIV的最佳分离是用正常人PMC而不是已建立的人细胞系实现的。
Isolates of HIV showed distinct differences in the ability to replicate in continuous human hematopoietic cell lines. Moreover, although all PMC cultures obtained from healthy individuals could be infected with HIV, considerable variation in the amount of virus released from different PMC cultures was observed. These biological properties of HIV could not be correlated with clinical state, binding properties of the virus isolates to target cells, or differences in target cell CD4 antigen expression. Some isolates of HIV that could not directly infect the HUT-78 cell line showed productive infection when PMC infected with these viruses were added to this human T cell line. These observations emphasize the importance of cell to cell contact in the spread of virus. The results demonstrate for the first time the differences in the host range specificity of HIV isolates in several individual PMC cultures, and indicate that the optimal isolation of HIV is achieved with normal human PMC rather than established human cell lines.