Metadherin is required for the proliferation, migration, and invasion of esophageal squamous cell carcinoma and its meta-analysis.
Metadherin is required for the proliferation, migration, and invasion of esophageal squamous cell carcinoma and its meta-analysis.
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DOI：
10.1016/j.trsl.2015.05.004
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发表时间：
2015-12
期刊：
影响因子：
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通讯作者：
Chenchen Yang;Shutao Zheng;Qing Liu;Tao Liu;Mang Lu;Fang Dai;Xiang-peng Gao;I. Sheyhidin;Xiaomei Lu
中科院分区：
文献类型：
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作者：
Chenchen Yang;Shutao Zheng;Qing Liu;Tao Liu;Mang Lu;Fang Dai;Xiang-peng Gao;I. Sheyhidin;Xiaomei Lu
Metadherin （MTDH）在各种鳞状细胞癌（SCCs）中高表达；然而，尚未进行评估MTDH与SCC相关性的meta分析。本研究旨在探讨MTDH在食管鳞状细胞癌（ESCC）中的生物学功能，并对MTDH与食管鳞状细胞癌的相关性进行meta分析。使用由86个ESCC和78对正常邻近组织（NATs）组成的ESCC组织阵列进行免疫组化检测MTDH的表达。与NATs相比，MTDH在ESCC组织中显著过表达，并与淋巴结转移、分化和预后显著相关。使用MTDH短发夹RNA质粒敲低MTDH可导致细胞周期阻滞在G0/G1期，并诱导EC9706细胞凋亡。敲低MTDH可抑制ESCC细胞的增殖、侵袭和迁移。此外，meta分析显示，MTDH过表达与SCC淋巴结转移、临床分期和组织T分类显著相关，提示MTDH可能作为ESCC淋巴结转移的潜在治疗靶点。
Metadherin (MTDH) was found to be highly expressed in various squamous cell carcinomas (SCCs); however, meta-analysis evaluating the association of MTDH in SCC has not been performed. The purpose of this study was to explore the biological functions of MTDH in esophageal squamous cell carcinoma (ESCC) and to meta-analyze the association between MTDH and SCC. Immunohistochemistry was performed to examine MTDH expression using an ESCC tissue array consisting of 86 ESCC and 78 paired normal adjacent tissues (NATs). MTDH was significantly overexpressed in ESCC tissues compared with NATs and was significantly associated with lymph node metastasis, differentiation, and prognosis. Knockdown of MTDH using an MTDH-short hairpin RNA plasmid caused cell cycle arrest at the G0/G1 phase and induced apoptosis of EC9706 cells. Knockdown of MTDH suppressed the proliferation, invasion, and migration of ESCC cells. Furthermore, meta-analysis revealed that overexpression of MTDH was significantly associated with the lymph node metastasis, advanced clinical stage, and T classification of tissues in SCC, suggesting that MTDH might be used as a potential therapeutic target in the lymph node metastasis of ESCC.