ISOLATION AND CHARACTERIZATION OF LYMPHOCYTES AND MACROPHAGES FROM SOLID, MALIGNANT HUMAN-TUMORS
ISOLATION AND CHARACTERIZATION OF LYMPHOCYTES AND MACROPHAGES FROM SOLID, MALIGNANT HUMAN-TUMORS
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DOI：
10.1002/ijc.2910230507
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发表时间：
1979-01-01
影响因子：
6.4
通讯作者：
SVAAR, H
中科院分区：
文献类型：
--
作者：
SVENNEVIG, JL;LOVIK, M;SVAAR, H
在17例人恶性实体瘤的机械制备细胞悬液中，发现0.5-5.0%(平均2.0%)的淋巴细胞和1.0-28.0%(平均7.4%)的巨噬细胞。用Boyum技术分离单个核细胞(MC)。每例活检重量为1-4克，平均1.3倍。淋巴细胞106个，0.8倍。回收巨噬细胞106个。对肿瘤浸润性淋巴细胞(TIL)进行T[胸腺来源细胞]和B[骨髓来源细胞]标记。与SRBC[绵羊红细胞](T细胞)形成TIL花环的比例为43%，显著低于癌症患者(58%)和正常对照(80%)的外周血淋巴细胞(PBL)。在TIL中，15%为B细胞，42%无T或B细胞标志。巨噬细胞(TIM)的鉴定采用非特异性酯酶染色和吞噬实验。4例肿瘤细胞同时进行α-丁酸萘酯染色。在4个肿瘤的低温切片上也有相应的发现。肿瘤组织内及周围可见巨噬细胞，偶见坏死区，但多数情况下周围细胞无坏死迹象。在一些肿瘤细胞悬液中可见典型的淋巴细胞和巨噬细胞群。癌症患者外周血中总淋巴细胞和T细胞计数明显降低，单核细胞总数在正常范围内。
In mechanically prepared cell suspensions from 17 solid, malignant human tumors, 0.5-5.0% (mean 2.0%) lymphocytes and 1.0-28.0% (mean 7.4%) macrophages were found. Mononuclear cells (MC) were isolated using the Boyum technique. From each biopsy weighing 1-4 g, on average, 1.3 .times. 106 lymphocytes and 0.8 .times. 106 macrophages were recovered. The tumor-infiltrating lymphocytes (TIL) were characterized with regard to T [thymus-derived cell] and B [bone marrow-derived cell] markers. The proportion of TIL-forming rosettes with SRBC [sheep red blood cells] (T cells) was 43%, significantly less than for peripheral blood lymphocytes (PBL) from cancer patients (58%) or normal controls (80%). Of the TIL 15% were B cells and 42% had no T or B cell markers. Macrophages (TIM) were identified by non-specific esterase staining and phagocytosis. In 4 cases the tumor cells were also stained with .alpha.-naphthyl butyrate. Corresponding findings were made on esterase-stained cryostat sections from 4 tumors. Macrophages were found within and around the tumor tissues, occasionally localized to necrotic areas, but in most cases with no sign of necrosis of the surrounding cells. In some tumor cell suspensions typical clusters of lymphocytes and macrophages were seen. Total lymphocyte and T cell counts were markedly reduced in the peripheral blood of cancer patients; total monocyte counts were within the normal range.