[Glycerol opening of blood-labyrinth barrier using electron-opaque tracer ferritin].
[Glycerol opening of blood-labyrinth barrier using electron-opaque tracer ferritin].
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发表时间：
1989
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通讯作者：
X. Ge
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作者：
X. Ge
将十五只豚鼠分为三组：对照组、铁蛋白组和甘油-铁蛋白组。第一组没有服用任何药物；第二组颈静脉注射铁蛋白1gm/kg。第三组在注射铁蛋白前30、60和120分钟注射50%甘油3ml/kg。去除皮纹，固定并嵌入 Epon 中。切割超薄切片并在电子显微镜下观察。对照组中没有看到铁蛋白分子。在铁蛋白组中，仅在毛细血管纹中看到铁蛋白颗粒。在甘油-铁蛋白组中，铁蛋白分子在30分钟时已进入毛细血管纹内皮细胞。 60分钟时，铁蛋白分子已进入血管纹并积聚在线粒体周围。不电子透明的示踪剂沿着微管系统迁移到边缘细胞的顶端，并通过细胞膜和连接处渗透到内淋巴空间。 120分钟时，铁蛋白颗粒出现在内淋巴间隙的边缘细胞表面。结果表明甘油可以作为血迷路屏障的开启剂。
Fifteen guinea pigs were divided into three groups: the control, the ferritin and the glycerol-ferritin groups. In the first group, no any medication had been given; in the second group, ferritin 1 gm/kg was injected via the jugular vein; in the third group, 50% glycerol 3 ml/kg was injected 30, 60 and 120 minutes prior to the injection of ferritin. Striae were removed, fixed and embedded in Epon. Ultra-thin sections were cut and observed under electron microscope. No ferritin molecule was seen in the control group. In the ferritin group, the ferritin particles were seen in stria capillary only. In the glycerol-ferritin group, the ferritin molecules had entered the endothelial cells of stria capillary at 30 minutes. Ferritin molecules had got into stria vascularis and accumulated around the mitochondria at 60 minutes. The electron-opaque tracers had migrated to the apex of the marginal cells along microtubular system and permeated to the endolymphatic space through cell membranes and junctions. The ferritin particles appeared at the surface of marginal cells in the endolymphatic space at 120 minutes. The results indicate that glycerol can serve as an opener of blood-labyrinth barrier.