EVALUATION OF CENTRIFUGATION CULTURES OF BRONCHOALVEOLAR LAVAGE FLUID FOR THE DIAGNOSIS OF CYTOMEGALO-VIRUS PNEUMONITIS
EVALUATION OF CENTRIFUGATION CULTURES OF BRONCHOALVEOLAR LAVAGE FLUID FOR THE DIAGNOSIS OF CYTOMEGALO-VIRUS PNEUMONITIS
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DOI：
10.1016/0732-8893(88)90092-2
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发表时间：
1988-08-01
影响因子：
2.9
通讯作者：
BALFOUR, HH
中科院分区：
文献类型：
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作者：
ERICE, A;HERTZ, MI;BALFOUR, HH
巨细胞病毒（CMV）肺炎是免疫功能低下患者中CMV疾病最严重的表现之一。CMV肺炎的诊断传统上需要使用侵入性操作，如肺活检。在这项回顾性研究中，我们评估了一种离心培养方法在样本的支气管肺泡液的CMV肺炎的非侵入性诊断。在9个月期间，收集了58例肺炎患者的75份支气管肺泡灌洗样本。我们分析了21例患者的数据，这些患者在支气管肺泡灌洗后14天内获得了肺组织样本。支气管肺泡液培养CMV阳性12例。在12例患者中的5例（42%）的肺组织样本中证实了CMV肺炎，而在其余7例（58%）病例中未发现CMV肺炎的证据。9例离心培养阴性的患者中，2例（22%）确诊为CMV肺炎。与传统培养相比，我们发现支气管肺泡灌洗液离心培养对CMV感染的检测具有高度敏感性（100%）和特异性（92%）。然而，通过离心培养检测CMV被证明是只有中等敏感性（71%）和非特异性（50%）诊断CMV肺炎。
Cytomegalovirus (CMV) pneumonitis is one of the most severe manifestations of CMV disease among immunocompromised patients. The diagnosis of CMV pneumonitis traditionally has required the use of invasive procedures such as lung biopsy. In this retrospective study, we evaluated a centrifugation culture method in samples of bronchoalveolar fluid for the non-invasive diagnosis of CMV pneumonitis. During a 9-mo period, 75 bronchoalveolar lavage samples were collected from 58 patients with pneumonitis. We analysed the data from 21 patients in whom lung tissue samples were obtained within 14 days of the bronchoalveolar lavage. Centrifugation cultures of bronchoalveolar fluid were positive for CMV in 12 cases. CMV pneumonitis was confirmed in samples of lung tissue from five (42%) of the 12 patients, whereas no evidence of CMV pneumonitis was found in the remaining seven (58%) cases. Of nine patients with negative centrifugation cultures, CMV pneumonitis was confirmed in two (22%). When compared with conventional cultures, we found bronchoalveolar lavage fluid centrifugation cultures to be highly sensitive (100%) and specific (92%) for the detection of CMV-infection. However, detection of CMV by centrifugation culture proved to be only moderately sensitive (71%) and nonspecific (50%) for the diagnosis of CMV pneumonitis.