PURIFICATION AND CHARACTERIZATION OF GROUP-A STREPTOCOCCAL PYROGENIC EXOTOXIN TYPE-C
PURIFICATION AND CHARACTERIZATION OF GROUP-A STREPTOCOCCAL PYROGENIC EXOTOXIN TYPE-C
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DOI：
10.1128/iai.16.2.673-679.1977
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发表时间：
1977-01-01
影响因子：
3.1
通讯作者：
WATSON, DW
中科院分区：
文献类型：
--
作者：
SCHLIEVERT, PM;BETTIN, KM;WATSON, DW
A组链球菌[化脓性链球菌] C型致热外毒素（SPE）通过在乙醇和乙酸盐缓冲盐水中的不同溶解度进行部分纯化。以这种方法制备的毒素由蛋白质和透明质酸组成。去除透明质酸后，该毒素仍具有致热原性，增加了家兔对致死性内毒素休克的敏感性，在酸、碱和胃蛋白酶处理下稳定，但热灭活。经薄层等电聚焦进一步纯化的毒素具有致热原性，并能增强家兔对致死性内毒素休克的敏感性。当通过Ouchterlony免疫扩散检测时，纯化的C型毒素呈现均一性，并且在等电聚焦聚丙烯酰胺凝胶（等电点，6.7）和十二烷基硫酸钠-聚丙烯酰胺凝胶（MW，13，200）中作为单一蛋白条带迁移。纯化的毒素在抗原性上不同于A和B SPE，并且针对纯化的毒素产生的抗血清中和热原活性。测定了氨基酸组成。
Group A streptococcal [Streptococcus pyogenes] pyrogenic exotoxin (SPE) type C was partially purified by differential solubility in ethanol and acetate-buffered saline. Toxin prepared in this way consisted of protein and hyaluronic acid. After removal of hyaluronic acid, the toxin remained pyrogenic, enhanced susceptibility of rabbits to lethal endotoxin shock, was stable when treated with acid, base or pepsin, but was inactivated by heat. Toxin further purified by thin-layer-isoelectric focusing was pyrogenic and enhanced the susceptibility of rabbits to lethal endotoxin shock. Purified type C toxin appeared homogeneous when tested by Ouchterlony immunodiffusion and migrated as a single protein band in isoelectric focusing polyacrylamide gels (isoelectric point, 6.7) and sodium dodecyl sulfate-polyacrylamide gels (MW, 13,200). The purified toxin was antigenically distinct from A and B SPE, and antisera raised against the purified toxin neutralized pyrogenic activity. The amino acid composition was determined.