Enriching the molecular definition of the airway "field of cancerization:" establishing new paradigms for the patient at risk for lung cancer.
Enriching the molecular definition of the airway "field of cancerization:" establishing new paradigms for the patient at risk for lung cancer.
复制标题
DOI：
10.1158/1940-6207.capr-12-0470
复制
发表时间：
2013-01
期刊：
影响因子：
--
通讯作者：
Dubinett SM
中科院分区：
文献类型：
--
作者：
Gomperts BN;Walser TC;Spira A;Dubinett SM
“癌化区域”是指与肿瘤组织相邻的组织学表现正常的组织，显示出分子异常，其中一些与肿瘤的异常相同。提高我们对这些分子事件的理解可能会增加我们对致癌作用的理解。在这里，卡达拉等人。尝试描述早期非小细胞肺癌（NSCLC）患者根治性手术后癌变领域在时间和空间上相关的分子事件。他们每年在肿瘤切除后对患者进行支气管镜检查，并从不同支气管内部位的连续刷检中提取 RNA。然后，他们进行了微阵列分析，以识别随时间推移和气道不同部位的基因表达差异。发现候选基因可能与癌化领域具有生物学相关性。例如，发现气道上皮中磷酸化 AKT 和 ERK1/2 的表达随着时间的推移而增加。尽管研究设计存在许多局限性，但这项研究证明了识别肺癌组织学正常气道上皮分子变化的实用性。除了增加我们对肺癌生物学的了解之外，研究癌化领域还有可能从以微创方式获得的样本中识别生物标志物。
The “field of cancerization” refers to histologically normal-appearing tissue adjacent to neoplastic tissue that displays molecular abnormalities, some of which are the same as those of the tumor. Improving our understanding of these molecular events is likely to increase our understanding of carcinogenesis. Here, Kadara et al. attempt to characterize the molecular events associated temporally and spatially within the field of cancerization of early stage non-small cell lung cancer (NSCLC) patients following definitive surgery. They followed patients with bronchoscopies annually after tumor resection and extracted RNA from the serial brushings from different endobronchial sites. They then performed microarray analysis to identify gene expression differences over time and in different sites in the airway. Candidate genes were found that may have biological relevance to the field of cancerization. For example, expression of phosphorylated AKT and ERK1/2 was found to increase in the airway epithelium with time. Despite a number of limitations in the study design, this investigation demonstrates the utility of identifying molecular changes in histologically normal airway epithelium in lung cancer. In addition to increasing our understanding of lung cancer biology, studying the field of cancerization has the potential to identify biomarkers from samples obtained in a minimally invasive manner.