Resveratrol fails to affect cocaine conditioned place preference behavior, but alleviates anxiety-like behaviors in cocaine withdrawn rats
Resveratrol fails to affect cocaine conditioned place preference behavior, but alleviates anxiety-like behaviors in cocaine withdrawn rats
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白藜芦醇不能影响可卡因条件性位置偏好行为，但可以减轻可卡因戒断大鼠的焦虑样行为
DOI：
10.1007/s00213-016-4210-4
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发表时间：
2016-04-01
影响因子：
3.4
通讯作者：
Guan, Xiaowei
中科院分区：
文献类型：
--
作者：
Hu, Panpan;Zhu, Wei;Guan, Xiaowei
目的观察白藜芦醇对可卡因戒断大鼠复发和焦虑样行为的影响，探讨白藜芦醇对大鼠海马区(HP)和前额叶皮质(PFC)白藜芦醇作用的分子机制。方法采用条件性位置偏爱(CPP)实验和高架+迷宫(EPM)实验分别检测大鼠条件位置偏爱(CPP)行为和焦虑样行为。给可卡因戒断大鼠注射白藜芦醇。检测血清丙二醛、谷胱甘肽和超氧化物歧化酶水平以评价氧化状态，测定IL-6、IL-1β和肿瘤坏死因子α水平以检测炎症状态，检测caspase-3和bax水平以评价幽门螺杆菌和门静脉癌变的细胞凋亡状态。结果白藜芦醇不影响可卡因戒断大鼠的CPP行为，但可减轻戒断大鼠的焦虑样行为。在可卡因戒断过程中，前额叶皮质丙二醛和肿瘤坏死因子α的表达水平以及幽门螺杆菌的丙二醛、超氧化物歧化酶、谷胱甘肽、白介素6、白介素1β、肿瘤坏死因子α、半胱氨酸天冬氨酸蛋白酶3和bax的表达水平均有显著变化，但两区的SIRT1表达无明显变化。除超氧化物歧化酶外，白藜芦醇可逆转可卡因戒断后上述神经化学改变。结论白藜芦醇对可卡因戒断大鼠的行为有一定的影响。Hp或PFC的氧化应激、炎症、细胞凋亡和SIRT1信号通路可能参与了RSV对可卡因戒断大鼠行为的调节作用。
RationaleResveratrol participates in regulating abnormal behaviors in psychostimulant-exposed animals.ObjectivesTo examine effects of resveratrol on relapse and anxiety-like behaviors in cocaine withdrawn rats and to investigate possible molecular mechanisms underlying resveratrol effects in hippocampus (HP) and prefrontal cortex (PFC).MethodsConditioned place preference (CPP) assay and elevated plus maze (EPM) test were used to examine cocaine CPP behavior and anxiety-like behaviors in rats, respectively. Resveratrol was administrated to cocaine withdrawn rats. Levels of MDA, GSH and SOD were examined to evaluate oxidative status, and levels of IL-6, IL-1β and TNF α were measured to examine inflammatory status and levels of caspase-3 and BAX was examined to evaluate apoptotic status in HP and PFC. SIRT expression was also examined here.ResultsResveratrol did not affect cocaine CPP behaviors, but attenuated anxiety-like behaviors in cocaine withdrawn rats. Levels of MDA and TNFα in PFC, and levels of MDA, SOD, GSH, IL-6, IL-1β, TNFα, caspase-3 and BAX in HP, but not SIRT1 expression in both regions were significantly changed during cocaine withdrawal period. Except SOD, resveratrol reversed above neurochemical changes induced by cocaine withdrawal. Furthermore, RSV induced a greater upregulation of SIRT1 expression in PFC in cocaine withdrawn rats than that in saline controls.ConclusionsCurrent findings suggest that resveratrol may influence behaviors in cocaine withdrawn rats. Oxidative stress, inflammation, apoptosis, and SIRT1 signaling pathway in HP or PFC might be involved in mediating effects of RSV on behaviors in cocaine withdrawn rats.