The Activity of a Small Lytic Peptide PTP-7 on Staphylococcus aureus Biofilms
The Activity of a Small Lytic Peptide PTP-7 on Staphylococcus aureus Biofilms
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DOI：
10.1007/s12275-011-1013-5
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发表时间：
2011-08-01
影响因子：
3
通讯作者：
Liang, Jun F.
中科院分区：
文献类型：
--
作者：
Kharidia, Riddhi;Liang, Jun F.
细菌生物膜最重要的特征之一是对抗生素和宿主免疫系统的抵抗力。在本研究中，我们发现了一种小分子裂解肽PTP-7，它对革兰氏阳性菌有很强的杀伤力，能够不分青红皂白地杀死对抗生素敏感和耐药的金黄色葡萄球菌。进一步的研究表明，尽管PTP-7是一种阳离子多肽，但其抗菌活性不受生物膜中带负电荷的胞外聚合物(EPS)的影响。PTP-7可以扩散到金黄色葡萄球菌生物膜的深层，高效杀灭生物膜内的细菌。金属离子的高浓度和生物膜中的酸性pH均不影响多肽PTP-7的活性。多肽PTP-7独特的序列/结构和抗菌活性使其具有抗生物被膜活性。本研究为细菌生物被膜的治疗，特别是各种生物被膜相关感染的治疗提供了新的思路。
One of the most important features of bacterial biofilms is their resistance to antibiotics and to the host immune system. In this study, we have found that a small lytic peptide, PTP-7, is very potent to Gram-positive bacteria and is able to kill antibiotic sensitive and resistant Staphylococcus aureus indiscriminately. Further studies have revealed that despite being a cationic peptide, the antibacterial activity of PTP-7 was not affected by the negatively charged extracellular polymeric substance (EPS) of biofilms. PTP-7 could diffuse into the deep layer of S. aureus biofilms to kill bacteria inside biofilms efficiently and effectively. Neither the high concentrations of metal ions nor the acidic pH in biofilms affected the activity of peptide PTP-7. It seems that the unique sequence/structure together with the resistant bacteria killing ability of peptide PTP-7 confers its anti-biofilm activity. This study sheds new light on the treatment of bacterial biofilms, especially various biofilm related infections.