The stimulatory effect of plasma samples from fattening cattle on adipogenesis related gene expression in preadipocyte cells
The stimulatory effect of plasma samples from fattening cattle on adipogenesis related gene expression in preadipocyte cells
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育肥牛血浆样本对前脂肪细胞脂肪生成相关基因表达的刺激作用
DOI：
10.1111/asj.12344
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发表时间：
2015
影响因子：
2
通讯作者：
Kouichi WATANABE and Hisashi ASO
中科院分区：
文献类型：
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作者：
Hitoshi WATANABE;Xiangning CHEN;Noriaki SHOJI;Ryo SAITO;Tatsuya NAKANO;Kazuki SAITO;Keisuke SUMIYOSHI;Michael T. ROSE;Natumi OKADA;Kouichi WATANABE and Hisashi ASO
生产含有高水平单不饱和脂肪酸（MUFA）的牛肉是可取的，因为这与脂肪的柔软性和适口性有关。然而，牛脂肪在增肥过程中MUFA合成的生理机制仍有待确定。在这项研究中，为了阐明血浆成分与肌内脂肪脂肪酸组成之间的关系，我们研究了从育肥牛获得的血浆对克隆牛肌内前脂肪细胞系（BIP）中脂肪生成相关基因mRNA表达的影响。从横膜不饱和脂肪酸与饱和脂肪酸比率（US/S）最高的牛的血浆样品中提取的血浆样品处理后，BIP细胞中硬脂酰辅酶a去饱和酶、脂肪细胞蛋白2、过氧化物酶体增殖体激活受体γ和固醇调节元件结合蛋白1的mRNA表达量显著增加。此外，血浆样品中非酯化脂肪酸（NEFA）浓度与胴体横膈膜US/S呈负相关。这些结果表明，通过测定血浆对BIP细胞基因表达的影响以及血浆中NEFA的浓度和组成，可以将脂肪US/S比较低的牛与屠宰前的育肥牛区分。
It is desirable to produce beef with high levels of monounsaturated fatty acids (MUFA), as this is related to fat softness and palatability. However, the physiology of MUFA synthesis in bovine fat during the fattening process remains to be established. In this study, in order to elucidate the relationship between plasma components and the fatty acid composition of intramuscular fat, we investigated the effect of plasma obtained from fattening cattle on the messenger RNA (mRNA) expressions of the adipogenesis‐related gene in a clonal bovine intramuscular preadipocyte line (BIP cells). The mRNA expressions of stearoyl‐CoA desaturase, adipocyte Protein 2, peroxisome proliferator‐activated receptor gamma and sterol regulatory element‐binding protein 1 in BIP cells were significantly higher following treatment with those plasma samples collected from the cattle with the highest diaphragmatic unsaturated fatty acids to saturated fatty acids ratio (US/S). Furthermore, the concentration of nonesterified fatty acid (NEFA) in the plasma samples had an inverse correlation with carcass diaphragmatic US/S. These results indicate that cattle with a low ratio of US/S in fat may be discriminated from the population of fattening cattle before slaughter by measuring the effect of their plasma on gene expression in BIP cells as well as their plasma concentration and composition of NEFA.