Construction of a Reduction‐responsive DNA Microsphere using a Reduction‐cleavable Spacer based on a Nitrobenzene Scaffold
Construction of a Reduction‐responsive DNA Microsphere using a Reduction‐cleavable Spacer based on a Nitrobenzene Scaffold
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使用基于硝基苯支架的还原可裂解间隔基构建还原响应 DNA 微球
DOI：
10.1002/asia.202200142
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Ikeda Masato
中科院分区：
文献类型：
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作者：
Higashi Sayuri L.;Isogami Ayaka;Takahashi Junko;Shibata Aya;Hirosawa Koichiro M.;Suzuki Kenichi G.N.;Sawada Shunsuke;Tsukiji Shinya;Matsuura Kazunori;Ikeda Masato
在这里，我们描述了基于硝基苯支架的一种新的还原可切割间隔（RCS）的设计和合成，用于根据标准磷酸酰胺化学构建还原反应性寡核苷酸。此外，我们证明了在寡核苷酸（30 nt）中间引入RCS可以构建自组装的微球，能够表现出还原响应性的拆卸。
Here, we describe the design and synthesis of a new reduction‐cleavable spacer (RCS) based on a nitrobenzene scaffold for constructing reduction‐responsive oligonucleotides according to standard phosphoramidite chemistry. In addition, we demonstrate that the introduction of the RCS in the middle of an oligonucleotide (30 nt) enables the construction of a self‐assembled microsphere capable of exhibiting a reduction‐responsive disassembly.