A newly formed hexaploid wheat exhibits immediate higher tolerance to nitrogen-deficiency than its parental lines.
A newly formed hexaploid wheat exhibits immediate higher tolerance to nitrogen-deficiency than its parental lines.
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DOI：
10.1186/s12870-018-1334-1
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发表时间：
2018-06-07
影响因子：
5.3
通讯作者：
Liu B
中科院分区：
文献类型：
--
作者：
Yang C;Yang Z;Zhao L;Sun F;Liu B
六倍体普通小麦(Triticum aestivum L.)与其四倍体小麦祖先相比，对多种胁迫环境具有更强的适应性。然而，普通小麦获得这些增强适应性的生理基础和进化过程仍未得到充分研究。在这里，我们的目的是调查普通小麦在异源六倍化后是否以及以什么方式表现出对低氮的耐性。比较了模拟天然普通小麦的人工合成异源六倍体小麦(neo-6×，BBAADD)及其四倍体(BBAA，4×)和二倍体(DD，2×)亲本的氮代谢相关性状。结果表明，在低氮条件下，NEO-6×与4×和2×亲本相比，光合作用基本保持正常，地上部氮素积累量较高，氮素同化能力较好。结果表明，neo-6×的耐氮性增强是多种机制共同作用的结果。在形态水平上，NEO-6×具有比亲本更高的根冠比，这可能是一种适应性生长策略，因为根更多地取食更少的氮素，从而使氮素在地上部的积累更高。在电生理水平上，neo-6×的H+外流高于其4×亲本。较强的H+外流可能导致较高的NEO-6×N吸收能力。在基因表达水平上，neo-6×显著高于其4×和2×亲本。本研究证明异源六倍体小麦比其4×亲本和2×亲本具有更高的耐氮性，这是通过多种机制实现的。本文的在线版本(10.1186/s12870-0181334-1)包含补充材料，可供授权用户使用。
It is known that hexaploid common wheat (Triticum aestivum L.) has stronger adaptability to many stressful environments than its tetraploid wheat progenitor. However, the physiological basis and evolutionary course to acquire these enhanced adaptabilities by common wheat remain understudied. Here, we aimed to investigate whether and by what means tolerance to low-nitrogen manifested by common wheat may emerge immediately following allohexaploidization. We compared traits related to nitrogen (N) metabolism in a synthetic allohexaploid wheat (neo-6×, BBAADD) mimicking natural common wheat, together with its tetraploid (BBAA, 4×) and diploid (DD, 2×) parents. We found that, under low nitrogen condition, neo-6× maintained largely normal photosynthesis, higher shoot N accumulation, and better N assimilation than its 4× and 2× parents. We showed that multiple mechanisms underlie the enhanced tolerance to N-deficiency in neo-6×. At morphological level, neo-6× has higher root/shoot ratio of biomass than its parents, which might be an adaptive growth strategy as more roots feed less shoots with N, thereby enabling higher N accumulation in the shoots. At electrophysiological level, H+ efflux in neo-6× is higher than in its 4× parent. A stronger H+ efflux may enable a higher N uptake capacity of neo-6×. At gene expression level, neo-6× displayed markedly higher expression levels of critical genes involved in N uptake than both of its 4× and 2× parents. This study documents that allohexaploid wheat can attain immediate higher tolerance to N-deficiency compared with both of its 4× and 2× parents, and which was accomplished via multiple mechanisms. The online version of this article (10.1186/s12870-018-1334-1) contains supplementary material, which is available to authorized users.
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影响因子：
6.9
作者：
Buchner P;Hawkesford MJ
通讯作者：
Hawkesford MJ
影响因子：
7.3
作者：
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通讯作者：
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影响因子：
4.3
作者：
Debouba, Mohamed;Gouia, Houda;Ghorbel, Mohamed Habib
通讯作者：
Ghorbel, Mohamed Habib
影响因子：
7.4
作者：
Saengwilai, Patompong;Nord, Eric A.;Lynch, Jonathan Paul
通讯作者：
Lynch, Jonathan Paul
影响因子：
4.8
作者：
Guo, Tiancai;Xuan, Hongmei;Kang, Guozhang
通讯作者：
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