A new model system for tomato genetics
A new model system for tomato genetics
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DOI：
10.1046/j.1365-313x.1997.12061465.x
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发表时间：
1997-12-01
期刊：
影响因子：
7.2
通讯作者：
Levy, A
中科院分区：
文献类型：
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作者：
Meissner, R;Jacobson, Y;Levy, A
本研究的目的是开发一个用于研究番茄遗传学的模式系统。文中呈现了农艺学、遗传学和分子学数据，这些数据表明小型番茄品种“Micro - Tom”（微型番茄）满足作为此类模式的要求。它能高密度种植（每平方米可达1357株）；生命周期短（从播种到果实成熟需70 - 90天）；并且通过农杆菌介导的子叶转化，其转化率可高达80%。此外，它与标准番茄品种仅相差两个主要基因。因此，任何突变或转基因都可在“Micro - Tom”的背景下方便地进行研究，并且在需要时可转移到标准背景中。我们利用“Micro - Tom”的特性来改进番茄诱变的基础设施。文中描述了对9000株M1代和20000株M2代EMS诱变植株的筛选。发现了色素沉着改变或叶片、花朵和果实形状改变的突变体。此外，基于玉米Ac/Ds转座元件的增强子捕获和基因捕获系统被转化到“Micro - Tom”中，并被发现是有活性的。总之，“Micro - Tom”为在番茄中实现饱和诱变开辟了新的前景，并促进了基于转座子的技术如基因标记、捕获和敲除的应用。
The purpose of this study was to develop a model system for studying tomato genetics. Agronomic, genetic, and molecular data are presented which show that the miniature Lycopersicon esculentum cultivar, Micro-Tom (Micro tomato), fulfills the requirements for such a model. It grows at high density (up to 1357 plants/m(-2)); it has a short life cycle (70-90 days from sowing to fruit ripening); and it can be transformed at frequencies of up to 80% through Agrobacterium-mediated transformation of cotyledons. Moreover, it differs from standard tomato cultivars by only two major genes. Therefore, any mutation or transgene can be conveniently studied in Micro-Tom's background and, when needed, transferred into a standard background. We took advantage of Micro-Tom's features to improve the infrastructure for mutagenesis in tomato. A screening of 9000 M1 and 20 000 M2 EMS mutagenized plants is described. Mutants with altered pigmentation or modified shape of leaves, flowers and fruits were found. In addition, an enhancer trapping and a gene trapping system, based on the Ac/Ds maize transposable elements, were transformed into Micro-Tom and found to be active. In summary, Micro-Tom opens new prospects to achieve saturated mutagenesis in tomato, and facilitates the application of transposon-based technologies such as gene tagging, trapping and knockout.