Nitric oxide induces transcriptional activation of the nitric oxide-tolerant alternative oxidase in Arabidopsis suspension cells
Nitric oxide induces transcriptional activation of the nitric oxide-tolerant alternative oxidase in Arabidopsis suspension cells
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DOI：
10.1007/s00425-002-0828-z
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发表时间：
2002-10-01
期刊：
影响因子：
4.3
通讯作者：
Durner, J
中科院分区：
文献类型：
--
作者：
Huang, X;von Rad, U;Durner, J
一氧化氮（NO）是一把双刃剑，它既可以是有益的，激活植物和动物的防御反应，也可以与活性氧一起杀死病原体和宿主。NO的主要靶点是细胞色素c依赖性呼吸。只有植物具有交替呼吸途径，交替氧化酶（AOX）作为终端电子受体。AOX已被认为是几乎没有受到NO的影响。在这里，我们表明，NO影响细胞色素依赖的呼吸在拟南芥（L.）Heynh..与此同时，通过使用cDNA微阵列和北方分析，用NO处理拟南芥细胞培养物实际上强烈诱导AOX 1a转录。根据转录积累，NO处理的悬浮细胞导致呼吸通过替代途径增加。向拟南芥细胞培养物中添加AOX抑制剂导致NO敏感性显著增加和细胞死亡。总之，我们的数据表明，NO诱导AOX1a基因，AOX可能参与抵消NO的毒性。本文的电子补充材料可以通过使用位于http：//www.example.com的Springer Link服务器获得。dx.doi.org/10.1007/sOO425-002-0828-z
Nitric oxide (NO) is a double-edged sword it can be either beneficial and activate defence responses in plants and animals or, together with reactive oxygen species, it can kill not only the pathogen but also the host. A prime target of NO is the cytochrome c-dependent respiration. Only plants possess alternative-pathway respiration with alternative oxidase (AOX) as a terminal electron acceptor. AOX has been suggested to be barely affected by NO. Here we show that NO affects cytochrome-dependent respiration in Arabidopsis thaliana (L.) Heynh.. At the same time, treatment of Arabidopsis cell cultures with NO actually strongly induced AOX1a transcription, as determined by using a cDNA microarray and by Northern analysis. In accordance with transcript accumulation, NO treatment of suspension cells resulted in increased respiration through the alternative pathway. Addition of an AOX inhibitor to Arabidopsis cell cultures resulted in dramatically increased NO-sensitivity and cell death. In all, our data suggest that NO induces the AOX1a gene and that AOX may participate to counteract the toxicity of NO. Electronic supplementary material to this paper can be obtained by using the Springer Link server located at http:// dx.doi.org/10.1007/sOO425-002-0828-z.