Protein extracts from cultured cells contain nonspecific serum albumin
Protein extracts from cultured cells contain nonspecific serum albumin
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培养细胞的蛋白质提取物含有非特异性血清白蛋白
DOI：
10.1080/09168451.2016.1151338
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Ohta S. *equal contribution
中科院分区：
文献类型：
--
作者：
Miyara M*;Umeda K*;Ishida K;Sanoh S;Kotake Y;Ohta S. *equal contribution
血清是细胞培养基的重要组成部分。本研究证明了细胞内蛋白提取物被培养基中的牛血清白蛋白污染，并说明了这种污染如何导致对western blot结果的误解。初步实验可以防止一些实验结果的误读，优化洗涤工艺可以实现特异性蛋白的检测。
Serum is an important component of cell culture media. The present study demonstrates contamination of intracellular protein extract by bovine serum albumin from the culture media and illustrates how this contamination can cause the misinterpretation of western blot results. Preliminary experiments can prevent the misinterpretation of some experimental results, and optimization of the washing process may enable specific protein detection.