A sequence-tagged linkage map of Brassica raga
A sequence-tagged linkage map of Brassica raga
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DOI：
10.1534/genetics.106.060152
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发表时间：
2006-09-01
期刊：
影响因子：
3.3
通讯作者：
Park, Beom-Seok
中科院分区：
文献类型：
--
作者：
Kim, Jung Sun;Chung, Tae Young;Park, Beom-Seok
构建了芜菁遗传连锁图谱，共包含545个标记位点，覆盖1287 cM，平均作图间隔为2.4cM。使用520个RFLP和25个基于PCR的标记的组合来鉴定这些位点。RFLP探针来源于359 B。11个B. rapa和26个拟南芥属。包括21个SSR标记提供了锚到以前发表的连锁图谱的B。rapa和B. napus的遗传作图，并作为R1-R10的参考作图。序列标记的标记允许解释B内的染色体复制模式。并与拟南芥进行比较。通过10个连锁群共定位了62个EST标记，表明这些标记可作为B染色体定位的锚定标记。拉帕。其它RFLP探针均扩增出2-5个位点，推测B.芜菁染色体组重复是一种通过10条染色体的普遍现象。该图谱包括FLC旁系同源物的五个位点，其代表先前报道的BrFLC-1、-2、-3和-5以及另外鉴定的源自R3上局部节段重复的BrFLC 3旁系同源物。
A detailed genetic linkage map of Brassica rapa has been constructed containing 545 sequence-tagged loci covering 1287 cM, with an average mapping interval of 2.4 cM. The loci were identified using a combination of 520 RFLP and 25 PCR-based markers. RFLP probes were derived from 359 B. rapa EST clones and amplification products of 11 B. rapa and 26 Arabidopsis. Including 21 SSR markers provided anchors to previously published linkage maps for B. rapa and B. napus and is followed as the referenced mapping of R1-R10. The sequence-tagged markers allowed interpretation of the pattern of chromosome duplications within the B. rapa genome and comparison with Arabidopsis. A total of 62 EST markers showing a single RFLP band were mapped through 10 linkage groups, indicating that these can be valuable anchoring markers for chromosome-based genome sequencing of B. rapa. Other RFLP probes gave rise to 2-5 loci, inferring that B. rapa genome duplication is a general phenomenon through 10 chromosomes. The map includes five loci of FLC paralogues, which represent the previously reported BrFLC-1, -2, -3, and -5 and additionally identified BrFLC3 paralogues derived from local segmental duplication on R3.