Targeted delivery of progenitor cells for cartilage repair
Targeted delivery of progenitor cells for cartilage repair
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DOI：
10.1016/j.orthres.2003.12.002
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发表时间：
2004-07-01
影响因子：
2.8
通讯作者：
Caplan, AI
中科院分区：
文献类型：
--
作者：
Dennis, JE;Cohen, N;Caplan, AI
已经在软骨缺损模型中测试了用于促进软骨形成祖细胞与特定基质分子粘附的方法。通过两步法包被用活体染料荧光标记的培养扩增的前软骨细胞，其中首先使脂化蛋白G嵌入细胞膜，第二次在软骨基质抗原的抗体溶液中孵育，使抗体与细胞外表面上的蛋白G结合。涂层技术（称为“细胞涂染”）不影响细胞活力或抑制生长和软骨形成潜力。然后将着色的细胞添加到具有部分厚度缺损的兔软骨外植体中，洗涤并准备用于组织学检查和共聚焦显微镜检查。组织学观察和共聚焦观察以及荧光强度定量一致地证明，涂有多种抗体的祖细胞能够优先结合到缺损内暴露的软骨基质。这些结果表明，用基质分子的抗体涂抹细胞膜是促进干细胞或祖细胞粘附到软骨损伤部位的有效方法。(C)2003骨科研究学会。由爱思唯尔有限公司出版。保留所有权利。
An approach for promoting the adherence of chondrogenic progenitor cells to specific matrix molecules has been tested in a cartilage defect model. Culture-expanded pre-chondrocytes fluorescently labeled with a vital dye were coated by a two-step method wherein lipidated protein G was first allowed to intercalate into cell membranes, and a second incubation in a solution of antibodies to cartilage matrix antigens allowed the binding of the antibodies to the protein G, on the external surface of the cell. The coating technique (termed "cell painting") does not effect cell viability or inhibit growth and chondrogenic potential. Painted cells were then added to rabbit cartilage explants that had a partial-thickness defect, washed, and prepared for histological examination and for confocal microscopy. The histological observations and the confocal observations and fluorescent intensity quantification consistently demonstrated that progenitor cells painted with multiple antibodies were capable of preferential binding to the exposed cartilage matrix within the defect. These results demonstrate that painting cell membranes with antibodies to matrix molecules is an effective method for promoting the adherence of stem or progenitor cells to a cartilage injury site. (C) 2003 Orthopaedic Research Society. Published by Elsevier Ltd. All rights reserved.