In vivo antimicrobial activity of marbofloxacin against Pasteurella multocida in a tissue cage model in calves.
In vivo antimicrobial activity of marbofloxacin against Pasteurella multocida in a tissue cage model in calves.
复制标题
马波沙星在犊牛组织笼模型中对多杀性巴氏杆菌的体内抗菌活性
DOI：
10.3389/fmicb.2015.00759
复制
发表时间：
2015
影响因子：
5.2
通讯作者：
Zeng Z
中科院分区：
文献类型：
--
作者：
Cao C;Qu Y;Sun M;Qiu Z;Huang X;Huai B;Lu Y;Zeng Z
麻保沙星是一种专门为兽医学开发的氟喹诺酮类药物，具有广谱抗菌活性。我们研究的目的是使用小牛皮下植入组织笼重新评估麻保沙星对多杀性巴氏杆菌的体内抗菌活性。小牛通过直接向组织笼内注射多杀性巴氏杆菌（B型，血清型2）感染，然后在接种24小时后肌肉注射一系列麻保沙星剂量。浓度 - 时间曲线下24小时面积与最小抑菌浓度或防突变浓度的比值（AUC24h/MIC或AUC24h/MPC）是通过非线性回归分析最能描述麻保沙星对多杀性巴氏杆菌有效性的药代动力学 - 药效学（PK/PD）指标（R² = 0.8514）。麻保沙星在体内表现出良好的抗菌活性。使用S形Emax模型WINNONLIN软件，产生50%（减少1.5 log₁₀CFU/mL）和90%（减少3 log₁₀CFU/mL）最大效应的AUC24h/MIC和AUC24h/MPC水平分别为18.60和50.65小时、4.67和12.89小时。通过组织笼模型的体内PK/PD综合方法在宿主防御存在的情况下，特别是在兽医学感兴趣的目标动物中，显示出评估抗菌活性和优化抗生素给药方案的优势。
Marbofloxacin is a fluoroquinolone specially developed for use in veterinary medicine with broad-spectrum antibacterial activity. The objective of our study was to re-evaluate in vivo antimicrobial activity of marbofloxacin against Pasteurella multocida using subcutaneously implanted tissue cages in calves. Calves were infected by direct injection into tissue cages with P. multocida(type B, serotype 2), then intramuscularly received a range of marbofloxacin doses 24 h after inoculation. The ratio of 24 h area under the concentration-time curve divided by the minimum inhibitory concentration or the mutant prevention concentration (AUC24 h/MIC or AUC24 h/MPC) was the pharmacokinetic-pharmacodynamic (PK/PD) index that best described the effectiveness of marbofloxacin against P. multocida (R2 = 0.8514) by non-linear regression analysis. Marbofloxacin exhibited a good antimicrobial activity in vivo. The levels of AUC24 h/MIC and AUC24 h/MPC that produced 50% (1.5log10 CFU/mL reduction) and 90% (3log10 CFU/mL reduction) of maximum response were 18.60 and 50.65 h, 4.67 and 12.89 h by using sigmoid Emax model WINNONLIN software, respectively. The in vivo PK/PD integrated methods by tissue cage model display the advantage of the evaluation of antimicrobial activity and the optimization of the dosage regimen for antibiotics in the presence of the host defenses, especially in target animal of veterinary interest.