Exogenous application of melatonin improves eradication of apple stem grooving virus from the infected in vitro shoots by shoot tip culture
Exogenous application of melatonin improves eradication of apple stem grooving virus from the infected in vitro shoots by shoot tip culture
复制标题
外源应用褪黑激素可通过茎尖培养提高受感染体外茎中苹果茎沟病毒的根除率
DOI：
10.1111/ppa.13018
复制
发表时间：
2019-06-01
期刊：
影响因子：
2.7
通讯作者：
Wang, Q. -C.
中科院分区：
文献类型：
--
作者：
Chen, L.;Wang, M. -R.;Wang, Q. -C.
生产和维护无病毒种植材料对于控制病毒性疾病至关重要。本研究试图测试外源应用褪黑激素从苹果品种 Gala 受病毒感染的体外芽中根除苹果茎沟病毒 (ASGV)。在芽增殖培养基中外源施用15μm褪黑素显着增加了芽的数量和芽长度。在含有15μm褪黑素的芽增殖培养基上增殖的芽中内源吲哚-3-乙酸(IAA)的水平最高。在此培养基上培养 4 周的病毒感染枝条的枝条再生水平显着高于对照。此外，病毒感染的离体枝条的茎尖在此培养基上增殖4周，结果95%的枝条脱毒，而在没有褪黑激素的情况下培养的病毒感染的枝条的枝尖则没有获得脱毒枝条。微组织直接RT-PCR和RT-qPCR分析表明，在含有15μm褪黑素的培养基上增殖4周的病毒感染芽的茎尖中ASGV浓度下降。病毒定位表明，外源应用褪黑激素扩大了病毒感染茎尖的无病毒区域。这些数据解释了为什么外源性应用褪黑激素可以有效根除 ASGV。外源施用褪黑素为根除植物病毒提供了一种替代手段，并具有生产无病毒植物的潜力。
Production and maintenance of virus-free planting materials is pivotal for the control of viral diseases. The present study attempted to test exogenous application of melatonin for eradication of apple stem grooving virus (ASGV) from virus-infected in vitro shoots of apple cultivar Gala. Exogenous application of 15 mu m melatonin to the shoot proliferation medium significantly increased the number of shoots and shoot length. The level of endogenous indole-3-acetic acid (IAA) was the highest in the shoots proliferating on the shoot proliferation medium containing 15 mu m melatonin. Shoot regrowth levels were significantly higher in shoot tips of the virus-infected shoots cultured for 4 weeks on this medium than the control. In addition, culture of shoot tips of the virus-infected in vitro shoots proliferated for 4 weeks on this medium resulted in 95% of shoots being virus-free, while no virus-free shoots were obtained in shoot tips of the virus-infected shoots cultured without melatonin. Analyses by microtissue direct RT-PCR and RT-qPCR showed that ASGV concentration decreased in shoot tips of the virus-infected shoots proliferating on the medium containing 15 mu m melatonin for 4 weeks. Virus localization showed that exogenous application of melatonin enlarged the virus-free area in the virus-infected shoot tips. These data provide explanations as to why exogenous application of melatonin can efficiently eradicate ASGV. Exogenous application of melatonin provides an alternative means for plant virus eradication and has the potential to produce virus-free plants.