ASSOCIATIONS OF HLA-DR AND HLA-DQ TYPES WITH ANTI-GBM NEPHRITIS BY SEQUENCE-SPECIFIC OLIGONUCLEOTIDE PROBE HYBRIDIZATION
ASSOCIATIONS OF HLA-DR AND HLA-DQ TYPES WITH ANTI-GBM NEPHRITIS BY SEQUENCE-SPECIFIC OLIGONUCLEOTIDE PROBE HYBRIDIZATION
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DOI：
10.1038/ki.1993.245
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发表时间：
1993-08-01
影响因子：
19.6
通讯作者：
WILSON, CB
中科院分区：
文献类型：
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作者：
HUEY, B;MCCORMICK, K;WILSON, CB
美国抗肾小球基底膜肾炎（Goodpasture综合征）患者23例，采用血清学方法分型HLAⅰ类和ⅱ类，采用序列特异性寡核苷酸（SSO）探针分型HLA- dr和HLA- dqb。使用的方案是第11届国际HLA研讨会的方案。我们能够证实DR2与疾病的强烈关联，并使用分子技术显示所有DR2患者仅限于DRB1-1501和DQB1-0602等位基因。我们还发现疾病与DRB1-0301和DQB1-0201等位基因之间存在高度显著的关联。DR4等位基因作为一个组在患者中并不明显更频繁，但等位基因DRB 1-0404确实与该疾病有关。SSO分型的使用为HLA和疾病相关等位基因的精细化分析提供了更多的机会。
Twenty-three patients from the United States with anti-glomerular basement membrane nephritis (Goodpasture's syndrome) were HLA class I and class II typed by serology and HLA-DR and HLA-DQB typed using sequence-specific oligonucleotide (SSO) probes. Protocols used were those of the 11th International HLA Workshop. We were able to confirm the strong association of DR2 with the disease and, using the molecular techniques, showed that all DR2 patients were restricted to DRB1-1501 and DQB1-0602 alleles. We also found highly significant associations between disease and the alleles of DRB1-0301 and DQB1-0201. DR4 alleles as a group were not significantly more frequent among patients, but the allele DRB 1-0404 did appear to be associated with the disease. The use of SSO typing provides an expanded opportunity for refined analysis of HLA and disease associated alleles.