Live Single-Cell Molecular Analysis by Video-Mass Spectrometry
Live Single-Cell Molecular Analysis by Video-Mass Spectrometry
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DOI：
10.2116/analsci.24.559
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发表时间：
2008-05
影响因子：
1.6
通讯作者：
N. Tsuyama;H. Mizuno;Emiko Tokunaga;T. Masujima
中科院分区：
文献类型：
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作者：
N. Tsuyama;H. Mizuno;Emiko Tokunaga;T. Masujima
在视频显微镜下对活单细胞的直接分子分析已经发展起来。将细胞内容物吸入纳米电喷雾针尖，采用纳米esi Q-TOF质谱法（MS）检测低分子量离子化合物的数百个峰。通过t检验提取单个小鼠胚胎成纤维细胞的细胞特异性MS峰，并对其中一个峰进行MS/MS分析进行分子鉴定。该方法可以直接、快速地通过视频显微镜同时观察细胞分子。
The direct molecular analysis of a live single cell viewed under a video-microscope has been developed. The cell contents are sucked into a nano-electrospray tip, and hundreds of peaks of ionic compounds of low molecular weight are detected by nano-ESI Q-TOF mass spectrometry (MS). Cell-specific MS peaks in a single mouse-embryonic fibroblasts cell are extracted by a t-test, and one of the peaks is proceeded to MS/MS analysis for molecular identification. This method is direct and quick to identify the molecules of a cell with simultaneous observation by a video-microscope.