IN-VITRO ASSESSMENT OF THE ETHANOL-INDUCED HEPATOTOXICITY ON HEPG2 CELL-LINE
IN-VITRO ASSESSMENT OF THE ETHANOL-INDUCED HEPATOTOXICITY ON HEPG2 CELL-LINE
复制标题
DOI：
10.1006/bbrc.1993.2569
复制
发表时间：
1993-12-15
影响因子：
3.1
通讯作者：
TIRIBELLI, C
中科院分区：
文献类型：
--
作者：
NEUMAN, MG;KOREN, G;TIRIBELLI, C
本研究报道了用人肝细胞系HepG2体外检测乙醇诱导的肝毒性。细胞在不断增加的乙醇浓度（10-80 mM）中孵育。通过四氮唑染料MTT的代谢和LDH和其他乙醇损伤标记酶（AST、GGT和GHD）释放到培养基中，分光光度法定量了细胞毒性。高达40 mM乙醇未观察到细胞毒性，而在较高浓度（60-80 mM乙醇）下发现剂量依赖性增加。在80 mM乙醇条件下，通过MTF和LDH释放评估，24小时后细胞存活率分别降至68%和60%（与对照组相比p <0.0001）。再暴露24小时不增加细胞毒性。透射电镜显示存在脂肪滴（脂肪变性）和线粒体损伤。所报道的方法似乎是一种有用的、可重复的技术，用于体外评估乙醇诱导的人肝细胞系的细胞毒性。
This study reports on measurement of the ethanol-induced hepatotoxicityin vitroon using the human hepatocyte cell line HepG2. Cells were incubated in the presence of increasing ethanol concentrations (10-80 mM). Cytotoxicity was quantitated spectrophotometrically both by the metabolism of the tetrazolium dye MTT and by the release into the medium of LDH and other marker enzymes of ethanol damage (AST, GGT and GHD). No cytotoxicity was observed up to 40 mM ethanol whereas a dose-dependent increment was found at higher concentrations (60-80 mM ethanol). At 80 mM ethanol, cell viability after 24 hours was reduced to 68% and 60% as assessed by MTF and LDH release respectively (p <0.0001 vs. controls). Exposure for additional 24 hours did not increase cytotoxicity. Transmission electron microscopy revealed the presence of fat droplets (steatosis) and mitochondrial damage. The method reported appears to be an useful and reproducible technique for thein vitroassessment of the ethanol-induced cytotoxicity in a human liver cell line.