Reactive Oxygen Species-Mediated DNA Damage and Apoptosis in Human Skin Epidermal Cells After Exposure to Nickel Nanoparticles
Reactive Oxygen Species-Mediated DNA Damage and Apoptosis in Human Skin Epidermal Cells After Exposure to Nickel Nanoparticles
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DOI：
10.1007/s12011-013-9871-9
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发表时间：
2014-01-01
影响因子：
3.9
通讯作者：
Al-Qahtani, Ahmed A.
中科院分区：
文献类型：
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作者：
Alarifi, Saud;Ali, Daoud;Al-Qahtani, Ahmed A.
纳米镍颗粒(NiNPs)由于其独特的性质，在各种领域得到了越来越广泛的应用。然而，关于NiNPs在人类皮肤细胞中的毒性的信息很少(A431)。本研究旨在探讨NiNPs对A431细胞的细胞毒性、细胞凋亡和DNA损伤作用。细胞增殖能力测试表明，NiNPs以剂量和时间依赖的方式诱导显著的细胞毒性。NiNPs还能诱导氧化应激，表现为产生活性氧(ROS)和谷胱甘肽(GSH)的耗竭。此外，抗氧化剂N-乙酰半胱氨酸(NAC)的共同处理减轻了由于NiNPs而产生的ROS，这表明了氧化应激的潜在机制。NiNPs还诱导A431细胞脂质过氧化、过氧化氢酶、超氧化物歧化酶和caspase-3活性显著升高。此外，NAC还可抑制NiNP诱导的caspase-3活性。用彗星试验进行的DNA片段化分析表明，NiNPs以剂量和时间依赖的方式引起遗传毒性。因此，这项研究指出了NiNPs诱导氧化应激导致细胞凋亡和遗传毒性的能力。这项研究需要在工业应用之前对NiNPs进行更仔细的评估。
Nickel nanoparticles (NiNPs) are increasingly used in various applications due to their unique properties. However, there is little information concerning the toxicity of NiNPs in the human skin cell (A431). The present study was designed to investigate the cytotoxicity, apoptosis, and DNA damage due to NiNPs in A431 cells. A cellular proliferative capacity test showed that NiNPs induce significant cytotoxicity in a dose-and time-dependent manner. NiNPs were also found to induce oxidative stress evidenced by the generation of reactive oxygen species (ROS) and depletion of glutathione (GSH). Further, co-treatment with the antioxidant N-acetylcysteine (NAC) mitigated the ROS generation due to NiNPs, suggesting the potential mechanism of oxidative stress. NiNPs also induced significant elevation of lipid peroxidation, catalase, and superoxide dismutase and caspase-3 activity in A431 cells. In addition, NAC suppressed NiNP-induced caspase-3 activity. DNA fragmentation analysis using the comet assay showed that the NiNPs cause genotoxicity in a dose-and time-dependent manner. Therefore, the study points out the capability of the NiNPs to induce oxidative stress resulting in apoptosis and genotoxicity. This study warrants more careful assessment of NiNPs before their industrial applications.