"One-step" Preparation of Thiol-Ene Clickable PEG-Based Thermoresponsive Hyperbranched Copolymer for In Situ Crosslinking Hybrid Hydrogel
"One-step" Preparation of Thiol-Ene Clickable PEG-Based Thermoresponsive Hyperbranched Copolymer for In Situ Crosslinking Hybrid Hydrogel
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DOI：
10.1002/marc.201100534
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发表时间：
2012-01-27
影响因子：
4.6
通讯作者：
Wang, Wenxin
中科院分区：
文献类型：
--
作者：
Dong, Yixiao;Saeed, Aram Omer;Wang, Wenxin
通过一锅一步失活增强原子转移自由基聚合方法合成了一种具有高丙烯酸乙烯基含量的明确聚乙二醇基超支化热响应共聚物，该方法通过与硫醇改性透明质酸生物聚合物的迈克尔型硫醇烯反应提供了可注射和原位可交联的体系。通过凝胶渗透色谱和1H NMR表征了共聚物的超支化结构、分子量和乙烯基含量百分比。该共聚物的下临界溶液温度接近体温，可在37℃下快速热凝胶化。交联水凝胶的扫描电子显微镜分析显示出多孔结构的网络形成，3D细胞培养研究表明细胞嵌入水凝胶后具有良好的细胞活力。这种可注射的原位交联混合水凝胶系统作为一种新型的组织工程混合生物材料具有广阔的前景。
A well-defined poly(ethylene glycol) based hyperbranched thermoresponsive copolymer with high content of acrylate vinyl groups was synthesized via a one-pot and one-step deactivation enhanced atom transfer radical polymerization approach, which provided an injectable and in situ crosslinkable system via Michael-type thiol-ene reaction with a thiol-modified hyaluronan biopolymer. The hyperbranched structure, molecular weight, and percentage of vinyl content of the copolymer were characterized by gel permeation chromatography and 1H NMR. The lower critical solution temperature of this copolymer is close to body temperature, which can result in a rapid thermal gelation at 37 degrees C. The scanning electron microscopy analysis of crosslinked hydrogel showed the network formation with porous structure, and 3D cell culture study demonstrated the good cell viability after the cells were embedded inside the hydrogel. This injectable and in situ crosslinking hybrid hydrogel system offers great promise as a new class of hybrid biomaterials for tissue engineering.