Effects of decabromodiphenyl ether and planting on the abundance and community composition of nitrogen-fixing bacteria and ammonia oxidizers in mangrove sediments: A laboratory microcosm study
Effects of decabromodiphenyl ether and planting on the abundance and community composition of nitrogen-fixing bacteria and ammonia oxidizers in mangrove sediments: A laboratory microcosm study
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十溴二苯醚和种植对红树林沉积物中固氮细菌和氨氧化剂丰度和群落组成的影响：实验室微观研究
DOI：
10.1016/j.scitotenv.2017.10.214
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发表时间：
2018
影响因子：
9.8
通讯作者：
Han Gao
中科院分区：
文献类型：
--
作者：
Juan Chen;Pei-Fang Wang;Chao Wang;Xun Wang;Han Gao
微生物固氮和氨氧化是两个重要的氮循环过程，但当沉积物受到持久性有机污染物污染时，驱动这两个过程的微生物如何反应却知之甚少。在这项研究中，我们进行了一个实验室微宇宙实验，以检查十溴联苯醚（BDE-209），无论是单独或与一个共同的红树林物种，白骨壤组合，在红树林沉积物中的固氮细菌（NFB）和氨氧化古菌（AOA）和细菌（AOB）的丰度，多样性和群落组成的影响。一年后，沉积物是非常有限的N。BDE-209污染的沉积物中的固氮速率和NFB丰度均显著高于未污染的沉积物，尤其是种植的沉积物，表明BDE-209和种植都促进了氮限制的红树林沉积物中的固氮。与此相反，BDE-209和种植的潜在硝化速率和AOA和AOB的丰度显着下降，并在沉积物与BDE-209的种植比在沉积物中的BDE-209或种植的抑制效果更强。焦磷酸测序结果表明，在BDE-209单独处理和BDE-209与种植联合处理的沉积物中，NFB的丰富度和多样性增加，AOA和AOB的丰富度和多样性降低。由于BDE-209单独或特别是与种植结合使用，沉积物中的非纤维蛋白、AOA和AOB的群落组成发生了显著变化，如变形菌门的一些非纤维蛋白增加、亚硝化菌属的AOA和亚硝化螺旋菌属的AOB分别减少所示。
While nitrogen (N) fixation and ammonia oxidation by microorganisms are two important N cycling processes, little is known about how the microbes that drive these two processes respond when sediments are contaminated with persistent organic pollutants. In this study, we carried out a laboratory microcosm experiment to examine the effects of decabromodiphenyl ether (BDE-209), either on its own or combined with a common mangrove species,Avicennia marina, on the abundance, diversity, and community composition of N-fixing bacteria (NFB) and ammonia-oxidizing archaea (AOA) and bacteria (AOB) in mangrove sediments. The sediments were very N-limited after one year. The rates of N fixation and NFB abundance were significantly higher in the sediments that contaminated by BDE-209, especially in the planted sediment, indicating that both BDE-209 and planting stimulated N fixation in N-limited mangrove sediments. In contrast, the potential nitrification rate and abundance of AOA and AOB decreased significantly under BDE-209 and planting, and the inhibitory effects were stronger in the sediment with both planting and BDE-209 than in the sediments with either BDE-209 or planting. The results from pyrosequencing showed that the richness and diversity of NFB increased, while those of AOA and AOB decreased, in the sediments treated with BDE-209 only and with BDE-209 combined with planting. The community compositions of NFB, AOA, and AOB in the sediments shifted significantly because of BDE-209, either alone or particularly when combined with planting, as shown by the increases in some NFB from the Proteobacteria phylum and decreases in AOA in theNitrosopumilusgenus and AOB in theNitrosospiragenus, respectively.